Team:Washington/Gram Positive/Test/MutantDataTable

From 2010.igem.org


Mutant Data Table

Mutant Data Table

Mutant Name Features and Mutations Transpeptidation per DCP Hydrolysis per DCP
CapD No Signal Sequence, Triple band on protein agarose gel 0.491 0.713
CapD_CP Foldit created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 1.000 1.000
CapD_CPNFDelta Foldit created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel, Removed "floppy" section to compact protein 1.035 0.878
F24A Point mutation at residue 24 on CapDCP from Phenylalanine to Alanine, Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.021 0.061
F24H Point mutation at residue 24 on CapDCP from Phenylalanine to Histidine, Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.108 1.205
F24H, L40R, T59D_M61S Point mutation at residues 24, 40, 59, and 61 on Foldit design from Phenylalanine to Histidine, Leucine to Arginine, Threonine to Asparagine, and Methionine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.001 0.006
F24H, L40R, T59N_M61S Point mutation at residues 24, 40, 59, and 61 on Foldit design from Phenylalanine to Histidine, Leucine to Arginine, Threonine to Aspartic Acid, and Methionine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.058 1.050
F24H, L40R, T59S_M61S Point mutation at residues 24, 40, 59, and 61 on CapDCP from Phenylalanine to Histidine, Leucine to Arginine, Threonine to Serine, and Methionine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.000 0.007
F24H, R356K Point mutation at residues 24 and 356 on CapDCP from Phenylalanine to Histidine and Arginine to Lysine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.129 0.257
F24H, T59R Point mutation at residues 24 and 59 on CapDCPfrom Phenylalanine to Histidine and Threonine to Arginine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.011 0.005
F24H,T59N Point mutation at residues 24 and 59 on CapDCP from Phenylalanine to Histidine and Threonine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.007 0.029
F24W Point mutation at residue 24 on CapDCP from Phenylalanine to Tryptophan. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.006 0.044
F24Y Point mutation at residue 24 on CapDCP from Phenylalanine to Tyrosine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.593 0.887
F24Y, L40R Point mutation at residues 24 and 40 on CapDCP from Phenylalanine to Tyrosine and Leucine to Arginine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.009 -0.013
F24Y, L40R, T59N Point mutation at residues 24, 40, 59 on CapDCP from Phenylalanine to Tyrosine, Leucine to Arginine,  and Threonine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.003 0.157
F24Y,R305K Point mutation at residues 24 and 305 on CapDCP from Phenylalanine to Tyrosine and Arginine to Lysine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.002 0.233
F24Y,T59N Point mutation at residues 24 and 59 on CapDCP from Phenylalanine to Tyrosine and Threonine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.008 0.165
F24Y,T59N,R305K Point mutation at residues 24, 59, and 356 on CapDCP from Phenylalanine to Tyrosine, Threonine to Asparagine, and Arginine to Lysine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.002 0.127
F60W Point mutation at residue 60 on CapDCP from Phenylalanine to Tryptophan. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.239 0.489
G79A Point mutation at residue 79 on CapDCP from Glycine to Alanine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.040 -0.511
I83T Point mutation at residue 83 on CapDCP from Isoleucine to Threonine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.009 0.006
I83V Point mutation at residue 83 on CapDCP from Isoleucine to Valine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.003 0.043
L40A Point mutation at residue 40 on CapDCP from Leucine to Alanine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.150 0.413
L40R Point mutation at residue 40 on CapDCP from Leucine to Arginine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 1.157 1.527
L40S Point mutation at residue 40 on CapDCP from Leucine to Serine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.166 0.577
L40W Point mutation at residue 40 on CapDCP from Leucine to Tryptophan. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.825 1.215
M61H Point mutation at residue 61 on CapDCP from Methionine to Histidine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel -0.003 0.002
M61N Point mutation at residue 61 on CapDCP from Methionine to Asparagine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.011 0.002
M61S Point mutation at residue 61 on CapDCP from Methionine to Serine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.262 0.485
N23K Point mutation at residue 23 on CapDCP from Asparagine to Lysine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.818 0.839
N23Q Point mutation at residue 23 on CapDCP from Asparagine to Glutamine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.165 0.646
S143K Point mutation at residue 143 on CapDCP from Serine to Lysine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.574 0.426
S22I, T59A Point mutation at residues 22 and 59 on CapDCP from Serine to Isoleucine and Threonine to Alanine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.030 -0.057
S22Q, T59Q Point mutation at residues 22 and 59 on CapDCP from Serine to Glutamine and Threonine to Glutamine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel -0.012 0.085
S57H, M61H Point mutation at residues 57 and 61 on CapDCP from Serine to Histidine and Methionine to Histidine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel -0.004 -0.007
T18L, T59Q, M61N, F452W Point mutation at residues 18, 59, 61, and 452 on CapDCP from Threonine to Leucine, Threonine to Glutamine, Methionine to Asparagine, and Phenylalanine to Tryptophan,  respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.020 -0.028
T18S_T20S Point mutation at residues 18 and 20 on CapDCP from Threonine to Serine and Threonine to Serine,  respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.617 1.186
T20A Point mutation at residue 20 on CapDCP from Threonine to Alanine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.011 0.021
T20C Point mutation at residue 20 on CapDCP from Threonine to Cystine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.090 0.142
T20S Point mutation at residue 20 on CapDCP from Threonine to Serine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.639 1.443
T20S, L40D Point mutation at residues 20 and 40 on CapDCP from Threonine to Serine and Leucine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.853 0.001
T20S, L40E Point mutation at residues 20 and 40 on CapDCP from Threonine to Serine and Leucine to Glutamic Acid, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.163 0.637
T20S, L40F Point mutation at residues 20 and 40 on CapDCP from Threonine to Serine and Leucine to Phenylalanine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.246 0.335
T20S, L40H Point mutation at residues 20 and 40 on CapDCP from Threonine to Serine and Leucine to Histidine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.001 0.012
T20S, L40K Point mutation at residues 20 and 40 on CapDCP from Threonine to Serine and Leucine to Lysine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.000 0.007
T20S, L40Q Point mutation at residues 20 and 40 on CapDCP from Threonine to Serine and Leucine to Glutamine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.126 0.478
T20S, L40R Point mutation at residues 20 and 40 on CapDCP from Threonine to Serine and Leucine to Arginine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.000 0.009
T20S, L40R, F60E Point mutation at residues 20, 40, and 60 on CapDCP from Threonine to Serine, Leucine to Arginine, and Phenylalanine to Glutamic Acid, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.048 0.028
T20S, L40R, F60Q Point mutation at residues 20, 40, and 60 on CapDCP from Threonine to Serine, Leucine to Arginine, and Phenylalanine to Glutamine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.002 0.007
T20S, L40R, N81E Point mutation at residues 20, 40, and 60 on CapDCP from Threonine to Serine, Leucine to Arginine, and Phenylalanine to Glutamine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.001 -0.004
T20S, L40R, N81Q Point mutation at residues 20, 40, and 81 on CapDCP from Threonine to Serine, Leucine to Arginine, and Asparagine to Glutamine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.422 0.321
T20S, L40R, T59K Point mutation at residues 20, 40, and 59 on CapDCP from Threonine to Serine, Leucine to Arginine, and Threonine to Lysine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.048 0.033
T20S, L40R, T59K_M61S Point mutation at residues 20, 40, 59, and 61 on CapDCP from Threonine to Serine, Leucine to Arginine, Threonine to Lysine, and Methionine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.010 0.032
T20S, T59Q, M61T Point mutation at residues 20, 59, and 61 on CapDCP from Threonine to Serine, Threonine to Glutamine, and Methionine to Threonine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.007 -0.069
T20S,M61A Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Alanine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.005 0.045
T20S,M61C Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Cystine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.102 0.207
T20S,M61D Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Aspartic Acid, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.004 0.003
T20S,M61G Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Glycine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.004 0.029
T20S,M61L Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Leucine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.004 0.029
T20S,M61N Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.003 0.002
T20S,M61S Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.007 0.053
T20S,M61T Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Threonine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.038 0.363
T20S,M61V Point mutation at residues 20 and 61 on CapDCP from Threonine to Serine and Methionine to Valine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.004 0.039
T20S,T59N Point mutation at residues 20 and 59 on CapDCP from Threonine to Serine and Threonine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.019 0.125
T20S,T59S_M61S Point mutation at residues 20, 59, and 61 on CapDCP from Threonine to Serine, Threonine to Serine, and Methionine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.300 0.719
T20S_F24H Point mutation at residues 20 and 24 on CapDCP from Threonine to Serine and Phenylalanine to Histidine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.008 0.097
T20S_F24Y Point mutation at residues 20 and 24 on CapDCP from Threonine to Serine and Phenylalanine to Tyrosine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.040 0.201
T20S_F24Y,T59N Point mutation at residues 20, 24, and 59 on CapDCP from Threonine to Serine, Phenylalanine to Tyrosine, and Threonine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.013 0.048
T2A Point mutation at residue 2 on CapDCP from Threonine to Alanine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.007 -0.020
T2C Point mutation at residue 2 on CapDCP from Threonine to Cystine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.001 -0.005
T2S Point mutation at residue 2 on CapDCP from Threonine to Serine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.024 0.325
T2S,T18S_T20S Point mutation at residues 2, 18, and 20 on CapDCP from Threonine to Serine, Threonine to Serine, and Threonine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.144 0.250
T2S,T18S_T20S,T59S_M61S Point mutation at residues 2, 18, 20, 59, and 61 on CapDCP from Threonine to Serine, Threonine to Serine, Threonine to Serine, Threonine to Serine and Threonine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.003 0.022
T2S,T20S Point mutation at residues 2 and 20 on CapDCP from Threonine to Serine and Threonine to Serine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.106 0.089
T2V Point mutation at residue 2 on CapDCP from Threonine to Valine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.000 0.000
T59H_M61A Point mutation at residues 59 and 61 on CapDCP from Threonine to Histidine and Methionine to Alanine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.000 0.000
T59H_M61T Point mutation at residues 59 and 61 on CapDCP from Threonine to Histidine and Methionine to Threonine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.000 0.000
T59M Point mutation at residue 59 on CapDCP from Threonine to Methionine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.008 0.008
T59N Point mutation at residue 59 on CapDCP from Threonine to Asparagine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.232 0.868
T59Q Point mutation at residue 59 on CapDCP from Threonine to Glutamine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.000 0.000
T59Q, M61Q, F452W Point mutation at residues 59, 61, and 452 on CapDCP from Threonine to Glutamine, Threonine to Glutamine, and Phenylalanine to Tryptophan, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel -0.014 0.037
T59S Point mutation at residue 59 on CapDCP from Threonine to Serine. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 3.525 1.047
T59S, M61H Point mutation at residues 59 and 61 on CapDCP from Threonine to Serine and Methionine to Histidine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel 0.013 0.051

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