Team:Washington/Team

Who we are

Undergraduate Team Members

Jeff Johnson	Chris Eiben	Tomas Huber
Ivan Huang	Peixian Liu	Denny Luan
Cindy Wu	Sean Wu	David Zong
Laura Austin	Matt Coyne	Justin De Leon
Matthew Harger	Jesa Landis

Graduate Advisors

Matt Smith Molecular and Cellular Biology	Ingrid Swanson Microbiology	Rob Egbert Electrical Engineering
Michal Galdzicki Biomedical and Health Informatics	Justin Siegel Biomolecular Structure and Design

Faculty Advisors

David Baker Biochemistry	Eric Klavins Electrical Engineering	Joseph Mougous Microbiology
Herbert Sauro Bioengineering

Collaborators and Support

Biochemistry Lab space	Microbiology Financial support and lab space	Fisher Lab Materials
Arthur Friedlander @ USAMRIID Help planning the project and offering to test our new enzymes' effectiveness against Anthrax!	Deepak Chandran @ Bioengineering, University of Washington Always available to answer our TinkerCell API questions no matter how trivial.

Who did what

The teams were assembled during our winter quarter. During this term we went around to classes and had weekly meeting to introduce students to synthetic biology through a series of guest lectures. During the spring students participated in brainstorming projects related to the sponsoring Faculty advisors labs (Baker, Mougous, Klavins, Sauro) and came up with the project they wanted to carry out. Over the summer, students completed all of the experimental work, but worked closely with both graduate students and faculty advisors to plan the most pertinent experiments and make the most of our limited time.

The Gram Positive Therapeutic

Chris Eiben, Sean Wu, Peixian Liu, David Zong, Ivan Huang, Cindy Wu, Denny Luan designed, expressed, purified, and characterized CapD, CapD_CP, and the 87 CapD_CP variants.

The Gram Negative Therapeutic

Matthew Harger, Matthew Coyne, and Jesa Landis cloned and characterized the Tse2/Tsi2 locus into BioBrick parts.

Laura Austin and Justin De Leon recombineered and characterized the T7 promoter into the T6SS fosmid.

New Tools

Jeff Johnson developed the WikiDust and PartsRobot software. Jeff also wrote BBF RFC 68. Michal Galdzicki and Professor Herbert M. Sauro read and revised the document.

Tomas Huber BioBricked the f1 origin, lacI repressor, and lacI regulated T7 promoter, taking them from the protein expression vector pET29b+ from Novagen. He also constructed and characterized 4 protein-based expression cassette's made from new AND old BioBricks.