Team:Imperial College London/Modelling/Signalling/Results and Conclusion

From 2010.igem.org

Revision as of 18:56, 25 October 2010 by Anita (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Department of Bioengineering

Division of Molecular Biosciences

Modelling	Overview \| Detection Model \| Signaling Model \| Fast Response Model \| Interactions
A major part of the project consisted of modelling each module. This enabled us to decide which ideas we should implement. Look at the Fast Response page for a great example of how modelling has made a major impact on our design!

Objectives | Description | Results | Constants | MATLAB Code

Results and Conclusion

Using this model, we can show that the phosphorylated ComE* is proportional to both initial concentration of AIP and ComD.

If the initial concentration of AIP or ComD is zero, there is no formation of ComE*. We are assuming an initial concentration of Phosphate and ComE of 100nM. If we change either [AIP]₀ or [ComE]₀, then the final concentration of ComE* will always tend towards 5×10^-11M. [ComE*]_final will always tend towards this value, unless the initial concentrations of Phosphate and ComE are changed. However, if we increase both [AIP]₀ and [ComE]₀ at the same time, then [ComE*]_final will be reached much faster (i.e. slope increases).

Graph showing how [ComE]_final eventually reaches the value 5×10^-11M.

1. Graph showing the production of Pr-ComD complex. 2. Graph showing the production of phosphorylated Pr-ComD* complex. 3. Graph showing the production of Pr-ComD*-ComE complex. Notice the steep increase of concentration for each of these graphs, which could be due to the high k_1,2,3,4 values.