Team:Newcastle/Meetings/21 October 2010

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• Apologies - Phil, Younus, Deena late
• Action point: Phil and Rachel add characteristation data to the wiki

Contents 1 yneA Characteristaion: 2 Arabinose 3 Concrete 4 Flux balance 5 Lab work 6 Safety page 7 Presentation 8 Other

yneA Characteristaion:

• ΔamyE:Pspac(hy)::yneA

• B. subtilis spread of IPTG may not be homogenous through the population.
• E. coli has transporter deltions: check E. coli wiki

• take out pMap65 results

• Median, Mode add to graph

• pMutin4 proportion of cells

• 20% responding- looks like

• Biomass of cells - length * amount (counts)) against IPTG

• Lysis acts to counter the overexpression of yneA ... so we have fewer cells

• Younus AP: send script for yneA to Jen, Matt, Neil

• Plot: Max cell length against ITPG + Error bars

Arabinose

• We think we have it cloned infront of GFP
• B. subtilis transformation tommorrow
• Fluorescence
• Characterisation of previous part!!!

Concrete

• Damaged building - earthquake

Flux balance

• difference between optimal and normal

Lab work

• DNA submitted... tracking number sent
• 1C3 SR1 has not arrived, we would have had 2 bricks submitted!

Safety page

• Deena and Harsh

• Risk assessment - Bacillus

• BioCOSH

• Safety Guy talk mention it

Presentation

• Phil - DON'T BE AGGRESSIVE!!!!

• Beginning:
  • say what the project is about
  • mention Bacillus subtilis
  • Why bacillus?
  • bioconcrete- show its current
  • Non-pathogenic not non-virulent
  • Previous work on CaCO₃
  • Don't use the word PROVES
  • Separate slid for work flows
  • Alan - Flux first, speak slowly
  • Summary table- redo
  • BRONZE, SILVER, GOLD!!!!!!!
• Gold
  • Jannetta write RFC
  • number on judging form
  • webservice!!!
• • Images are ours

Other

• Insurance form
• Taxi
• Younus - Poster next thursday due in?
• Action point check presentation and poster submission!!!