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Team:Newcastle/Meetings/21 October 2010
From 2010.igem.org
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- Apologies - Phil, Younus, Deena late
- Action point: Phil and Rachel add characteristation data to the wiki
Contents |
yneA Characteristaion:
- ΔamyE:Pspac(hy)::yneA
- B. subtilis spread of IPTG may not be homogenous through the population.
- E. coli has transporter deltions: check E. coli wiki
- take out pMap65 results
- Median, Mode add to graph
- pMutin4 proportion of cells
- 20% responding- looks like
- Biomass of cells - length * amount (counts)) against IPTG
- Lysis acts to counter the overexpression of yneA ... so we have fewer cells
- Younus AP: send script for yneA to Jen, Matt, Neil
- Plot: Max cell length against ITPG + Error bars
Arabinose
We think we have it cloned infront of GFP Bacillus subtilis transformation tommorrow Fluorescence Characterisation of previous part!!!
Concrete
- Damaged building - earthquake
Flux balance
- difference between optimal and normal
Lab work
DNA submitted... tracking number sent 1C3 SR1 has not arrived, we would have had 2 bricks submitted!
Safety page
Deena and Harsh
Risk assessment- Bacillus
BioCOSH
Safety Guy talk mention it
Presentation
Phil DON'T BE AGGRESSIVE!!!!
Beginning:
- say what the project is about
- mention Bacillus subtilis
- Why bacillus?
- bioconcrete- show its current
- Non-pathogenic not non-virulent
- Previous work on CaCO3
- Don't use the word PROVES
- Separate slid for work flows
- Alan - Flux first, speak slowly
- Summary table- redo
- BRONZE, SILVER, GOLD!!!!!!! (see below)
GOLD
Jannetta write RFC
- number on judging form
- webservice!!!
SEM
Images are ours
Other
Insurance form Taxi
Younus - Poster next thursday due in?
Action point check presentation and poster submission!!!
   
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