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Team:KIT-Kyoto/Parts
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We use these parts for making original biobrick parts
1.We get these parts from 2010 iGEM kit
| Name | Part type | Resistance | Insert | Vector | Contents |
|---|---|---|---|---|---|
| <partinfo>pSB3k3</partinfo>(<partinfo>BBa_J04450</partinfo>) | Plasmid backbone | Kanamycin | 738bp | 2750bp | promoter(LacI) +RBS+mRFP+T+T |
| <partinfo>pSB6A1</partinfo>(<partinfo>BBa_J04450</partinfo>) | Plasmid backbone | Ampicillin | 738bp | 4032bp | promoter(LacI) +RBS+mRFP+T+T |
| <partinfo>pSB1C3</partinfo>(<partinfo>BBa_J04450</partinfo>) | Plasmid backbone | Chloramphenicol | 1069bp | 2072bp | promoter(LacI) +RBS+mRFP+T+T |
| <partinfo>pSB1A2</partinfo>(<partinfo>BBa_I3522</partinfo>) | Composite | Ampicillin | 937bp | 2079bp | promoter(TetR)+RBS+GFP+T+T |
| <partinfo>pSB1A2</partinfo>(<partinfo>BBa_R0040</partinfo>) | Regulatory | Ampicillin | 54bp | 2079bp | promoter(TetR) |
| <partinfo>pSB1A2</partinfo>(<partinfo>BBa_I732019</partinfo>) | Regulatory | Ampicillin | 3230bp | 2079bp | RBS+LacZ+T+T |
| <partinfo>pSB1AK3</partinfo>(<partinfo>BBa_I732950</partinfo>) | Protein domain | Kanamycin | 3230bp | 2079bp | RBS+LacZ+T+T |
| <partinfo>pSB4A5</partinfo>(<partinfo>BBa_K193602</partinfo>) | Composite | Ampicillin | 1896bp | 3395bp | |
| <partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0240</partinfo>) | Protein domain | Ampicillin | 883bp | 2079bp | RBS+GFP+T+T |
| <partinfo>pSB1A2</partinfo>(<partinfo>BBa_I13507</partinfo>) | Protein domain | Ampicillin | 937bp | 2079bp | RBS+RFP+T+T |
| <partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0420</partinfo>) | Protein domain | Ampicillin | 878bp | 2079bp | RBS+CFP+T+T |
| <partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0430</partinfo>) | Protein domain | Ampicillin | 878bp | 2079bp | RBS+YFP+T+T |
| <partinfo>pSB1A2</partinfo>(<partinfo>BBa_I13600</partinfo>) | Composite | Ampicillin | 940bp | 2079bp | promoter(TetR)+RBS+CFP+T+T |
| <partinfo>pSB1A2</partinfo>(<partinfo>BBa_J22005</partinfo>) | Composite | Ampicillin | 2079bp | 2623bp | promoter(TetR)+RBS+YFP+T+T |
2.We get this part directly from iGEM HQ
We characterize this BioBrick Part and enter the new information back on the Registry. This parts don’t include detailed information on pSB6 and the reasons leading to its usage in our work to produce GFP. In general, pSB6 is a low copy plasmid which makes it more suitable for protein production than the high copy vector. However this information cannot be found in any sites related to iGEM. Consequently, we tried to compare the performances of the high copy vector pSB1 and low copy vector pSB6. We have confirmed in this way that pSB6 is more efficient at producing GFP protein than PSB1. We have thus proved that this part is very useful to evaluate the capabilities of a promoter, in this case by observing the degree of fluorescence of GFP protein. We are very thankful to the 09’Tokyo-Tech group who has supplied us with this part.
>>[pSB1C3]
| Name | Part Type | Resistance | Insert | Vector | |
|---|---|---|---|---|---|
| <partinfo>pSB6A1</partinfo>(<partinfo>BBa_K121013</partinfo>) | Protein domain | Ampicillin | 883bp | 4022bp | RBS+GFP+T+T |
We make or design these parts originally.
We transformed these inserts into the vector,pSB6A1.And we transformed 2 inserts(BBa_K362005,BBa_K362007) of them into pSB1C3.
<groupparts>iGEM010 KIT-Kyoto</groupparts>
