Minnesota-experimental/2 March 2010

From 2010.igem.org

Revision as of 19:34, 23 October 2010 by Adams696 (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Rachel Used PCR purification kit to purify digestion reaction. Performed ligation reaction with pucmod BB BglII/NotI vector and Eut MN BglII/NotI insert. Reaction was a 25 microliter reaction. Tony and Matt Concentrated Eut LK solution in speed-vac for sixteen minutes. Eut LK solution digested with BglII/NotI (50 microliter reaction). Rachel and Ian Transformation of 15 microliters of ligation reaction from 3/2. Plated on amp plates at 37 degree overnight.

Retrieved from "http://2010.igem.org/Minnesota-experimental/2_March_2010"