User contributions
From 2010.igem.org
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- 08:45, 24 October 2010 (diff | hist) N Minnesota-experimental/13 October 2010 (New page: '''Matt''' Sent J23105 and J23118 into pSBLC3 plasmids for sequencing at University of Minnesota BioMedical Genomics Center.) (top)
- 08:44, 24 October 2010 (diff | hist) N Minnesota-experimental/11 October 2010 (New page: '''Matt''' Preparations of transformations for FACS protein assay.) (top)
- 08:43, 24 October 2010 (diff | hist) N Minnesota-experimental/8 October 2010 (New page: '''Matt''' Ran gel to confirm digestion band. Transformation of J23105 and J23118 into Top 10 competency cells. Fifty microliters of each plated onto CM plates at thirty seven degree ove...) (top)
- 08:42, 24 October 2010 (diff | hist) N Minnesota-experimental/7 October 2010 (New page: '''Ian''' Digestion reactions of pSBK3 pLac GFP. PCR confirms DNA band sizes.) (top)
- 08:40, 24 October 2010 (diff | hist) N Minnesota-experimental/6 October 2010 (New page: '''Matt''' Minipreps of J23105 and J23118 after cell cultures. Given to Poonam for transformations. Digestion reactions for confirmation with EcoRI/PstI. Placed in thirty degree incubat...) (top)
- 08:38, 24 October 2010 (diff | hist) N Minnesota-experimental/4 October 2010 (New page: '''Ian''' Minipreps of psBLC3 eut SK. Digestion reactions of pSBLC3 with EcoRI/PstI. PCR reactions of pSBLC3 eut SK. pSBLC3 pLac GFP sent for sequencing to University of Minnesota BioMe...) (top)
- 08:35, 24 October 2010 (diff | hist) Minnesota-experimental/2 October 2010 (top)
- 08:35, 24 October 2010 (diff | hist) N Minnesota-experimental/2 October 2010 (New page: '''Matt''' Re-do minipreps of J23105 and J23118 promoters.)
- 08:34, 24 October 2010 (diff | hist) N Minnesota-experimental/1 October 2010 (New page: '''Ian''' Minipreps of Eut SK insert. Ligation reactions of insert into PsBLC3. Transformation reactions of PsBLC3 Eut SK. Stored overnight at thirty seven degrees Celsius incubator.) (top)
- 08:33, 24 October 2010 (diff | hist) N Minnesota-experimental/30 September 2010 (New page: '''Matt''' Mininpreps of overnight culture colonies. Digestion of minipreps with EcoRI/PstI. Overnight in thirty seven degree incubator. '''Tony''' Inoculated JM109 cultures in liquid LB...)
- 08:31, 24 October 2010 (diff | hist) N Minnesota-experimental/29 September 2010 (New page: '''Tony''' No colonies observed. Second attempt of transformations from 9/28. '''Matt''' Transformations of J23105 and J23118 promoters.) (top)
- 08:30, 24 October 2010 (diff | hist) N Minnesota-experimental/28 September 2010 (New page: '''Tony''' Transformations of pucBB EutS, MN, LK, MNLK, SMNLK and pacBB GFP.) (top)
- 08:29, 24 October 2010 (diff | hist) Minnesota-experimental/27 September 2010 (top)
- 08:28, 24 October 2010 (diff | hist) N Minnesota-experimental/27 September 2010 (New page: '''Matt''' PCR of 23102 and 23116 promoters.)
- 08:27, 24 October 2010 (diff | hist) N Minnesota-experimental/22 September 2010 (New page: '''Ian''' Digestion reaction failed. Attempted with PCR. Also ligation reaction with Eut SK into PsBLC3.) (top)
- 08:23, 24 October 2010 (diff | hist) N Minnesota-experimental/21 September 2010 (New page: '''Ian''' Minipreps of psBLC3 Eut SK overnight cultures. Digestion raction of psBLC3 Eut SK with EcoRI/PstI. '''Matt''' Digestion reaction of J23102 and J23116 with EcoRI/SpeI. Placed in...) (top)
- 08:19, 24 October 2010 (diff | hist) N Minnesota-experimental/20 September 2010 (New page: '''Matt''' PCR reaction of J23102 and J23116 promoters. Gel confirms correct DNA band size. .) (top)
- 08:16, 24 October 2010 (diff | hist) Minnesota-experimental/17 September 2010 (top)
- 08:15, 24 October 2010 (diff | hist) N Minnesota-experimental/17 September 2010 (New page: '''Matt''' Performed minipreps of J23102 and J23116 plasmids.)
- 08:14, 24 October 2010 (diff | hist) N Minnesota-experimental/16 September 2010 (New page: '''Tony''' S, M, N, L, K digestions with BglII/NotI and EcoRI/PstI. Ligation of S, M, N, L, K into PsBLC3 linearized plasmid.) (top)
- 08:13, 24 October 2010 (diff | hist) N Minnesota-experimental/15 September 2010 (New page: '''Annie''' Inoculated overnight cultures of psB3K pLac into LB. Transfered two of the inoculations to two new LB tubes.) (top)
- 02:04, 24 October 2010 (diff | hist) Minnesota-experimental/14 September 2010 (top)
- 02:04, 24 October 2010 (diff | hist) N Minnesota-experimental/14 September 2010 (New page: '''Tony''' Ran S, M, N, L, K, SMNLK, and GFP out on gel. Miniprep plux with elution in fifty microliters EB. Digestion of plux with EcoRI and SpeI.)
- 02:02, 24 October 2010 (diff | hist) N Minnesota-experimental/13 September 2010 (New page: '''Matt''' Transformations of BBa J23102 and BBa J23116. '''Tony''' Inoculated plux cultures overnight.) (top)
- 02:01, 24 October 2010 (diff | hist) N Minnesota-experimental/8 September 2010 (New page: '''Tony''' Transformations of pucBB EutS, M, N, L, K and EutSMNLK and pucBB GFP.) (top)
- 02:01, 24 October 2010 (diff | hist) N Minnesota-experimental/7 September 2010 (New page: '''Ian Made new competent cells. CAT ligase free cloning with pucBB CAT BglII/NotI. Also made fifteen ampicillin plates.) (top)
- 01:57, 24 October 2010 (diff | hist) N Minnesota-experimental/2 September 2010 (New page: '''Ian''' PCR of CAT product and PCR of pichia genomic DNA.) (top)
- 01:56, 24 October 2010 (diff | hist) N Minnesota-experimental/31 August 2010 (New page: '''Tony''' PCR reactions of AOX and CAT from Pichia genomic DNA.) (top)
- 01:55, 24 October 2010 (diff | hist) N Minnesota-experimental/30 August 2010 (New page: '''Ian''' Tested competent cells w/pucBB GFP. Ran PCR and gel of AOX and CAT.) (top)
- 01:36, 24 October 2010 (diff | hist) N Minnesota-experimental/25 August 2010 (New page: '''Ian''' Ran digest of puc BB AOX with BglII/NotI. Gel confirms that fragment is not the correct size) (top)
- 01:32, 24 October 2010 (diff | hist) N Minnesota-experimental/24 August 2010 (New page: '''Ian''' PCR reaction in order to check presence of AOX gene.) (top)
- 01:31, 24 October 2010 (diff | hist) N Minnesota-experimental/23 August 2010 (New page: '''Ian''' Ligation of Eut SK and psB1C3 (twenty microliter reaction).) (top)
- 01:30, 24 October 2010 (diff | hist) N Minnesota-experimental/21 August 2010 (New page: '''Ian''' PCR purification of digest miniprep of AOX ligation.) (top)
- 01:30, 24 October 2010 (diff | hist) N Minnesota-experimental/20 August 2010 (New page: '''Matt''' Overnight cultures of pucBB-AOX colonies. '''Ian''' Ran digest of Eut SK PCR with EcoRI/PstI and ran out on successful gel.) (top)
- 01:27, 24 October 2010 (diff | hist) N Minnesota-experimental/18 August 2010 (New page: '''Ian''' Ran digest of psB3K3 Eut SK with PstI/EcoRI. Ran successful PCR and gel of digest.) (top)
- 01:26, 24 October 2010 (diff | hist) N Minnesota-experimental/17 August 2010 (New page: '''Matt''' Ligation of AOX and CAT genes into pucBB.) (top)
- 01:25, 24 October 2010 (diff | hist) N Minnesota-experimental/16 August 2010 (New page: '''Matt''' Transformation of Pichia AOX and CAT. No reaction observed. Digestion of AOX and CAT PCR DNA with BglII/NotI and placed in thirty seven degree overnight. '''Tony''' PCR for DA...) (top)
- 01:23, 24 October 2010 (diff | hist) N Minnesota-experimental/6 August 2010 (New page: '''Tony''' Spun down colonies and stored two of them in freezers. No visible gel result from colony PCR reaction.) (top)
- 01:22, 24 October 2010 (diff | hist) N Minnesota-experimental/5 August 2010 (New page: '''Tony and Matt''' Ran gel of AOX and CAT genes. Reaction successful. Also ran colony PCR in order to confirm appearance of pucBB-DAS colonies. '''Ian''' PCR reaction of psB3K3 pLac-GFP...) (top)
- 01:14, 24 October 2010 (diff | hist) N Minnesota-experimental/4 August 2010 (New page: '''Tony''' Nanodrop concentrations of pucBB NotI/BglII and DAS PCR product. Ligase free cloning of DAS pCR product and pucBB BglII/NotI. Second attempt at PCR in order to isolate AOX and...) (top)
- 01:10, 24 October 2010 (diff | hist) N Minnesota-experimental/3 August 2010 (New page: '''Tony and Matt''' Ran gel of PCR resutls. Successfully amplified the DAS genes but still failed to isolated CAT genes and AOX genes. '''Ian''' Minipreps of psB3K3 pLac GFP.) (top)
- 01:01, 24 October 2010 (diff | hist) N Minnesota-experimental/2 August 2010 (New page: '''Matt and Tony''' PCR reaction of Pichia genes AOX, CAT and DAS. Used positive control EutG GFP.) (top)
- 00:58, 24 October 2010 (diff | hist) N Minnesota-experimental/31 July 2010 (New page: '''Ian''' Transformation of pLac and GFP/psB3K3 ligation. Sent psB3K3 eut SK, BglII Eut MN-F for sequencing to University of Minnesota BioMedical Genomics Center.) (top)
- 00:56, 24 October 2010 (diff | hist) N Minnesota-experimental/29 July 2010 (New page: '''Ian''' Ran digest of pLac with EcoRI/SpeI.) (top)
- 00:55, 24 October 2010 (diff | hist) N Minnesota-experimental/28 July 2010 (New page: '''Annie''' Ran gel of previous day PCR. Inoculated Pichia from Pichia plate in YPD and kept in thirty degree shaker. '''Ian''' Ran gel of GFP fragment (XbaI/PstI). Transformation of psB...) (top)
- 00:52, 24 October 2010 (diff | hist) N Minnesota-experimental/27 July 2010 (New page: '''Annie''' Test gel run of PCR products. PCR of genomic DNA (reaction failed). '''Tony''' Third attempt at PCR of Pichia genes (AOX, CAT, and DAS). '''Ian''' Miniprep of overnight cultur...) (top)
- 00:49, 24 October 2010 (diff | hist) N Minnesota-experimental/26 July 2010 (New page: '''Tony and Matt''' PCR reactions of AOX, DAS, and CAT.) (top)
- 00:48, 24 October 2010 (diff | hist) Minnesota-experimental/22 July 2010 (top)
- 00:39, 24 October 2010 (diff | hist) Minnesota-experimental/22 July 2010
- 00:39, 24 October 2010 (diff | hist) N Minnesota-experimental/22 July 2010 (New page: '''Annie''' Genomic extraction of pichia. '''Mat''' Transformation of pacBB EutG DsRed ligation in thirty seven degree incubator.)
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