Team:Washington/Team

Who we are

Undergraduate Team Members

Jeff Johnson Sauro Team Member	Chris Eiben Baker Team Member	Tomas Huber Baker Team Member
David Zong Baker Team Member	Sean Wu Baker Team Member	Ivan Huang Baker Team Member
Cindy Wu Baker Team Member	Denny Luan Baker Team Member	Laura Austin Mougous Team Member
Matthew Harger Mougous Team Member	Matt Coyne Mougous Team Member	Jesa Landis Mougous Team Member
Justin De Leon Mougous Team Member	Peixian Liu Baker Team Member

Graduate Advisors

Matt Smith Molecular and Cellular Biology	Ingrid Swanson Microbiology	Rob Egbert Electrical Engineering
Michal Galdzicki Biomedical and Health Informatics	Justin Siegel Biomolecular Structure and Design

Faculty Advisors

David Baker Biochemistry	Joeseph Mougous Microbiology	Eric Klavins Electrical Engineering
Herbert Sauro Bioengineering

Collaborators and Support

Biochemistry Lab space	Microbiology Financial support and lab space	Fisher Lab Materials
Arthur Friedlander @ USAMRIID Help planning the project and offering to test our new enzymes on their effectiveness against Anthrax!

Who did what

The teams was assembled during our winter quarter. During this team we went around to classes and had weekly meeting to introduce students to synthetic biology through a series of guest lectures. During the spring students participated in brainstorming projects related to the sponsoring Faculty advisors labs (Baker, Mougous, Klavins, Sauro) and came up with the project they wanted to carry out. Over the summer students completed all of the experimental work, but worked closely with both graduate student and faculty advisors in order to plan the most pertinent experiments in order to make the most of our limited amount of time.

The Gram Positive Therapeutic

Chris Eiben, Sean Wu, Peixian Liu, David Zong, Ivan Huang, Cindy Wu, Denny_Luan expressed, purified, and characterized capD, capD_cp, and the 87 capD_cp variants.

The Gram Negative Therapeutic

Matthew Harger, Matthew Coyne, and Jesa Landis cloned and characterized the Tse2/Tsi2 locus into BioBrick parts. Laura Austin and Justin De Leon recombineered and characterized the T7 promoter into the T6SS fosmid.

New Tools

Jeff Johnson developed the WikiDust and PartsRobot software Tomas Huber BioBrick'ed the an f1 origin, lacI repressor, and lacI regulated T7 promoter. He also constructed and characterized 4 based protein expression cassette's made up of new AND old BioBricks.