| Photos
|
Logos
|
|
| Our Imperial College iGEM Logo
|
|
|
| PARASIGHT
|
|
|
| Another really cool logo
|
|
The Team
|
|
| The team with advisors Chris and Tom
|
|
|
| The Imperial College iGEM Team
|
|
|
| Some team members with SynBio icon Jay Keasling!!!
|
|
|
| Maddie and a bit of Kirill in labs
|
|
|
| Maddie in labs again
|
|
|
| Nick
|
|
|
| Maddie in labs... AGAIN
|
|
|
| Kirill, Maddie and Nick
|
|
|
| Nick (posing?)
|
|
|
| Piotr focusing, Anita not so much!
|
|
|
| Piotr and Anita
|
|
|
| Maddie and Kirill
|
|
|
| Anita and Ben
|
|
|
| Nick knows ONE song on the guitar! Wow
|
|
|
| Anja, Kyasha and Florian
|
|
|
| Check out Piotr's laser eyes
|
|
Brainstorming
|
|
| Fv-fragment guided input module
|
|
|
| Ideas on parasite detection
|
|
|
| Nick's idea on cell population control
|
|
|
| Nick's idea...
|
|
|
| Nick's idea...
|
|
|
| Nick's idea...
|
|
Wet Lab
|
Surface Protein
|
|
| Surface Protein Team Logo
|
|
|
| Assembly Strategy for LytC
|
|
|
| Gel confirming that DNA (pSB1C3 and pSB1AK3 with BOO14) has been digested properly by PstI and EcoRI.
|
|
|
| Gel of PCR confirming pSB1C3 has been amplified.
|
|
|
| Picture estimating the concentration of purified BOO14 and pSB1C3.
|
|
|
| Colony PCR to detect sucessfully transformed bacteria.
|
|
|
| 3 PCR reactions to determine the most effective protocol to amplify the CWB.
|
|
|
| Gel of a diagnostic digest of the vector confirming that the terminator has been inserted.
|
|
|
| Isolation of the promoter pVEG by gel purification.
|
|
|
| Determining the ratio of pSB1C3 to pVEG for ligation.
|
|
|
| Determining the ratio of lytC to pSB1C3 for ligation.
|
|
Vectors
|
|
| Vectors Team Logo
|
|
|
| Assembly strategy for the AmyE vector
|
|
|
| Assembly strategy for the PyrD vector
|
|
|
| K14002 and oligo assembly
|
|
|
| K143070 and K11064 assembly step
|
|
XylE
|
|
| XylE Team Logo
|
|
|
| Team XylE
|
|
|
| The J23101 gene in a biobrick vector containg RFP gene.
|
|
|
|
| Midi-prep XylE digestion with xbaI and PstI
|
|
|
| Gel analysis of XylE, J23101 promoter and pSB1C3 vector samples
|
|
|
| Catechol assay (before)
|
|
.jpg)
|
| Catechol assay (after) - Can you spot the difference?
|
|
|
| Catechol assay on XylE-trasformed cells in a 96-well plate
|
|
|
| First Successful Catechol Assay!
|
|
|
| First Successful Catechol Assay!
|
|
|
| :)
|
|
|
| Test confirming that yellow product of XylE enzmymatic reaction is leaks back from the cytosol into the solution.
|
|
|
| The coloured pigment is transported out of the cells.
|
|
|
| Spectra of XylE transformed E.coli after addition of catechol assay
|
|
|
| Catechol Assay cuvette setup
|
|
|
| Catechol Assay cuvette setup
|
|
Strategy
|
|
| The Engineering Design Cycle
|
|
|
| The full assembly strategy
|
|
|
| Wolf's primers
|
|
Parts
|
|
| BioBricks
|
|
|
| ComE binding sites in the promoters of the comCDE and comAB operons
|
|
Detection Module
|
|
| Dr Martha Betson explaining the Schistosoma lifecycle
|
| | |
|
| Detection Module Construct
|
|
|
| Surface Protein Construct
|
|
|
| Detection Module Schematic
|
|
Signalling Module
|
|
| Results of a BLAST search using ComE (Accession no. AAC44897)
|
|
|
| Results of a BLAST search using BlpR (Accession no. CAC03515.1)
|
| |
| | |
| | |
| | |
| |
|
| Signalling Module Construct
|
|
|
| Signalling Module Pathway
|
|
Fast Response Module
|
| | |
|
| Fast Response Module Construct
|
|
|
| Fast Response Module Testing Construct
|
|
Human Practices
|
|
| Human Practices panel: advisors
|
|
|
| The team looks reeeeaaaalllly interested
|
|
|
| Maddie's notes from the HP panel
|
|
|
| Notes...
|
|
|
| Notes...
|
|
|
| Should scientists be allowed to create artificial life?
|
|
|
| Should scientists be allowed to create artificial life?
|
|
|
| Building prototypes informed our design
|
|
|
| We wanted our kit to be as useful as possible
|
|
School Workshops
|
|
| Brainstorming
|
|
|
| Chameleon: Now you see me - now you don't
|
|
|
| More brainstorming
|
|
|
| ...and more brainstorming
|
|
|
| Ideas on regulation of Synthetic Biology
|
|
|
| H'n'H sauce (Hot&Healthy) - yummy
|
|
|
| More ideas on regulations of SynBio
|
|
|
| Brainstorming again
|
|
|
| iGEM <3
|
|
|
| Check out the lens creating bacteria!
|
|
|
| More ideas...
|
|
|
| Happy Bacteria?!
|
|
|
| Regulations...
|
|
"
