Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14
From 2010.igem.org
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Contents |
2010/10/14
1:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
2:DNA extraction
<Member>
nito
<Sample>
<Protocol>
See Protocol 4
3:DNA Ligation
<Member>
nito
<Sample>
(after digestion)
<Protocol>
See Protocol 10
4:DNA Digestion
<Member>
nito
<Sample, Materials>
・PCR productions
- plasmid
・Digest enzyme
- XbaⅠ
- SpeⅠ
<Protocol>
See Prptpcpl 9
5:PCR
<Member>
nito
<Sample>
<Protocol>
See Protocol 1
6:DNA extraction from gels
<Member>
nito
<Sample>
(After electrophoresis)
<Protocol>
SeeProtocol 4
7:DNA Digestion
<Member>
nito
<Sample, Materials>
・PCR productions
・Digest enzyme
- AvrⅡ
- NheⅠ
- XbaⅠ
- SpeⅠ
<Protocol>
See Prptpcpl 9
8:PCR
<Member>
nito
<Sample>
<Protocol>
See Protocol 1
9:DNA extraction from gels
<Member>
nito
<Sample>
- 9-7
(After electrophoresis)
<Protocol>
SeeProtocol 4
10:Electrophoresis
<Member>
nito
<Sample>
<Protocol>
See Protocol 8
11:DNA extraction from gels
<Member>
nito
<Sample>
(After electrophoresis)
<Protocol>
SeeProtocol 4