Notebook

First we were organized the work in teams.

The WetLab Team is finally divided in Production Team and Assembly Team, while our previous teams are still active: Sensing Team (David Caballero and Eva Fernández) and Chemotaxis-Adhesion Team(Adrián Arellano, Paola Gallardo and Félix Reyes). The WetLab team is supervised by Fernando Govantes.

• Production Team
  

  Components: David Caballero and Paola Gallardo. Each component carries out individual work.

  Goal: Biobrick synthesis by PCR and SDM (site-directed mutagenesis) using E. coli K12 (wild type) DNA as a template. Parts to be amplified are fecA*, fecR*, fecI, PfecA, gltB, gltD**. Asterisk means presence of restriction targets: *PstI or **PstI and EcoRI.

• Assembly Team
  

  Components: Adrián Arellano, Félix Reyes and Eva Fernández.

  Goal: Building of biobrick circuits mainly by 3A Assembly and Standard Assembly.

• Assay Team
  

  Components: Félix Reyes and David Caballero.

  Goal: Testing the final project as well as all the biobriks that have been produced by Assembly Team.