Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/03
From 2010.igem.org
Contents |
2010/10/03
1:PCR
<Member>
hito
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 2
2:DNA extraction
<Member>
mio
<Sample>
PCR products
<Protocol>
See Protocol 4
3:Electrophoresis
<Member>
nito
<Sample>
All PCR products(10/3)
<Protocol>
See Protocol 8
4:DNA extraction
<Member>
mio,mizuki,nito
<Sample>
PCR products
Digestion products
<Protocol>
See Protocol 4
5:DNA Ligation
<Member>
mio,mizuki
<Sample>
(after digestion)
<Protocol>
See Protocol 10
6:Transformation
<Member>
nito
<Sample>
- 7-8-10
- JM109
<Protocol>
See Protocol 7
7:DNA Digestion
<Member>
nito
<Sample, Materials>
・PCR productions
<Protocol>
See Prptpcpl 9