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From 2010.igem.org

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Contents 1 2010/09/29 1.1 1：DNA extraction 1.2 2:Electrophoresis 1.3 3：DNA Ligation 1.4 4:PCR

2010/09/29

1：DNA extraction

＜Member＞
nito

＜Sample＞
PCR products

• 1 (9/27)①
• 1 (9/27)②
• 2 (9/27)
• 3 (9/27)
• 4 (9/27)①
• 4 (9/27)②
• 5(9/27)
• 8 (9/27)
• 10(9/27)

Digestion products (9/27)

• 1
• 2
• 4
• 5

＜Protocol＞
See Protocol 4

2:Electrophoresis

<Member>
nito

<Sample>
PCR products (9/27)

• 1
• 2
• 3
• 4

<Protocol>
See Protocol 8

<Result>

3：DNA Ligation

＜Member＞
nito

＜Sample＞

• 1,2
• 4,5

(after digestion)

＜Protocol＞
See Protocol 3

4:PCR

<Member>
mio,mizuki

<Sample>(temperature in annealing:℃)

• 5 (70)
• 6 (63)
• 7-8(70)
• 9 (58,63,69,70,72)
• 10 (58)
• 1-2(72)

<Protocol>
See Protocol 1
But today we used Kod.