Project Outline

Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology. The Aim is to Simulate Antibody Selection and Production Technology with Bacteria. Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody. In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:

MODULE I: Generation of Antibody Library

MODULE II: Selection of Specific Antibody

Module I

Abstract

Flow chart

Landing Pad Insertion

Helper Plasmid(HP) Insertion

Donor Plasmid(DP) Construction

Strategy 1

Strategy 2

Donor Plasmid(DP) Insertion & Recombination Induction

Removal of Helper Plasmid(HP)

Module II

Strategy 1

Strategy 2

Strategy 3

Cooperation with Macquarie Australia