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Lab notes (9/20 - 9/26)

Contents 1 Lab notes (9/20 - 9/26) 1.1 Flagella Group 1.1.1 Restriction Digest of FlhDCmut-B0015 and pSB3T5 with J13002 1.1.2 Ligation of pSB3T5 with J13002 and FlhDCmut-B0015 1.1.3 Transformation of FlhDCmut-B0015 inserted into pSB3T5 with J13002

Flagella Group

Restriction Digest of FlhDCmut-B0015 and pSB3T5 with J13002

Date: 9/25 2010
Done By: Louise, Pernille and Sheila
Protocol: RD1.1 and DE1.3
Notes:
Results: gel extraction
Conclusion:

Ligation of pSB3T5 with J13002 and FlhDCmut-B0015

Date: 9/25 2010
Done By: Louise, Pernille and Sheila
Protocol: LG1.2
Notes: Samples were prepared in the ratios 1:1, 1:2 and 1:4
Results:
Conclusion:

Transformation of FlhDCmut-B0015 inserted into pSB3T5 with J13002

Date: 9/26 2010
Done By: Louise and Sheila
Protocol: CC1.1 and TR1.1
Notes:
Results: Can be seen here and here.
Conclusion:

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