Team:Freiburg Bioware/NoteBook/Labjournal/March

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1. Labday 31.03.2010

Sequence analysis

Investigators: Christian W., Bea, Kira, Achim, Kerstin, Hanna, Christian L. (Tobias, Kristian)


Theoretical cloning


Sequence analysis (Alignement) of the Stratagene Vector with

  • AAV2
  • AAV5
  • AAV7
  • AAV8

result: Media:AAV_alignment.pdf
Curiously there were some significant differences in the aligned sequences of several AAV genomes resulting in frame shifts. Maybe because the sequences are from 1983 and therefore incomplete. In case of doubt we count on the Stratagene-sequence.

To do:

  • download and compare of further AAV serotypes. (In Geneious)
  • compare literature
  • find out which possibilities are given to modify the Capsid

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