Team:Newcastle/7 September 2010

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Contents

Subtilin Immunity BioBrick

Aims

The aim of this experiment is to perform minipreps of the 16 overnight cultures that were left overnight from yesterday for the Subtilin Immunity BioBrick.

Methods

The Qiagen Miniprep protocol was used for each of the 16 cultures, followed by the NanoDrop Spectrophotometer protocol.

Results, Discussion and Conclusion

The nanodrop spectrophotometer results for the 16 minipreps for Subtilin Immunity are below (units: ng/ml):

Tube Nanodrop Value (in ng/ml)
1 76.5
2 61.5
3 84.6
4 19.5
5 89.9
6 87.6
7 6.3
8 69.5
9 81.3
10 74.4
11 68.5
12 70.2
13 83.5
14 77.6
15 73.3
16 63.0


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