Team:Newcastle/Meetings/4 June 2010

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Informal Meeting - 4 June 2010

Accessory genes and nickel incorporation necessary more complicated than we thought.
Software engineering cycle
Requirements -> specification-> design-> implementation -> testing -> maintenance ...link back to the requirements
Specification more detail on the requirements- literature
Urease calcium carbonate, optimise for Bacillis
Increase expression- what we thought!
Biosynthesis of active BS in the Absence of Known Urease Accessory Proteins: paper
Accessory genes required!
Look over papers from tutorial
Sporosarcina pasteurii
Biobrick site symbols
Promoter-ribosome- binding- coding sequence –ribosome binding site-coding-ribosome binding coding- terminator sequences ...and spacers
pSpac
ncbi – find the genome – can see the genes
Implementation using sequencher
Find the sequence of part
Assemble parts
Biobrick friendly restriction sites
Genbank/embl file format
Implementation altered by cloning strategy
Biobricks RFC- request for comments
[http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_10:_Draft_standard_for_Biobrick_biological_parts duplo and lego don’t fit together]
[http://www.youtube.com/watch?v=FRijFX0BpGI&feature=player_embedded Video for lego pipette]

(Others modelling Filamentous and spidersilk killswitch)

(Others Modelling glue and lysis quorum sensing)

Group 1 starts 14th June: SW, D.Y AK, (HS); working on Biobrick 2 & 5 (End of crack & signalling system; Glue & lysis)

Group 2 starts 21st June: JS, RB, RC, PH, (YE); working on BioBrick 3 & 4 (Non-target-environment kill switch; Spider silk and filamentous cells)

Team members not in the lab training will be working on modeling.
HS and YE will unfortunately be away for both lab training sessions, hence the brackets