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Revision as of 17:03, 26 October 2010 by
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TEAM ALBERTA
HOME
AT A GLANCE
PROJECT
Overview
BioBytes 2.0
Software
Modelling
The Kit
Kit Components
ACHIEVEMENTS
Overview
Medal Requirements
Media
HUMAN PRACTICES
Overview
Distribution Analysis
High School
DiscoverElle Visit
Safety
PARTS
TIMELINE & PROTOCOLS
Overview
General Protocols
Reusable Plates
Base Plasmids
Creating Parts
Transforming Cells
Anchor
Beads
Optimizations
Software
TEAM
Contents
1
List of General Protocols
List of General Protocols
Number
Title
1
Transformations
2
5mL Overnight
3
Glycerol Stock
4
Plasmid Miniprep
5
Restriction Digest
6
Agarose Gel Electrophoresis
7
Gel Extraction
8
Ligation
9
Colony PCR
10
PCR Purification
11
Sample Labelling Conventions
12
Vector Dephosphorylation
13
Fluorescent Sequencing Reaction
14
LB Plates and Broth
15
Primer Design
16
PCR
17
In Vitro BioByte Assembly