Team:KIT-Kyoto/Parts

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We used these parts for making our original biobrick parts

1.We obtained these parts from 2010 iGEM kit

NamePart typeResistanceInsertVectorContents
<partinfo>pSB3k3</partinfo>(<partinfo>BBa_J04450</partinfo>)Plasmid backboneKanamycin738bp2750bppromoter(LacI) +RBS+mRFP+T+T
<partinfo>pSB6A1</partinfo>(<partinfo>BBa_J04450</partinfo>)Plasmid backboneAmpicillin738bp4032bppromoter(LacI) +RBS+mRFP+T+T
<partinfo>pSB1C3</partinfo>(<partinfo>BBa_J04450</partinfo>)Plasmid backboneChloramphenicol1069bp2072bppromoter(LacI) +RBS+mRFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I3522</partinfo>)CompositeAmpicillin937bp2079bppromoter(TetR)+RBS+GFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_R0040</partinfo>)RegulatoryAmpicillin54bp2079bppromoter(TetR)
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I732019</partinfo>)RegulatoryAmpicillin3230bp2079bpRBS+LacZ+T+T
<partinfo>pSB1AK3</partinfo>(<partinfo>BBa_I732950</partinfo>)Protein domainKanamycin3230bp2079bpRBS+LacZ+T+T
<partinfo>pSB4A5</partinfo>(<partinfo>BBa_K193602</partinfo>)CompositeAmpicillin1896bp3395bp
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0240</partinfo>)Protein domainAmpicillin883bp2079bpRBS+GFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I13507</partinfo>)Protein domainAmpicillin937bp2079bpRBS+RFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0420</partinfo>)Protein domainAmpicillin878bp2079bpRBS+CFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_E0430</partinfo>)Protein domainAmpicillin878bp2079bpRBS+YFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_I13600</partinfo>)CompositeAmpicillin940bp2079bppromoter(TetR)+RBS+CFP+T+T
<partinfo>pSB1A2</partinfo>(<partinfo>BBa_J22005</partinfo>)CompositeAmpicillin2079bp2623bppromoter(TetR)+RBS+YFP+T+T

2.We obtained this part directly from iGEM HQ

We characterize this BioBrick Part and enter the new information back on the Registry. This parts don’t include detailed information on pSB6 and the reasons leading to its usage in our work to produce GFP. In general, pSB6 is a low copy plasmid which makes it more suitable for protein production than the high copy vector. However this information cannot be found in any sites related to iGEM. Consequently, we tried to compare the performances of the high copy vector pSB1 and low copy vector pSB6. We have confirmed in this way that pSB6 is more efficient at producing GFP protein than PSB1. We have thus proved that this part is very useful to evaluate the capabilities of a promoter, in this case by observing the degree of fluorescence of GFP protein. We are very thankful to the 09’Tokyo-Tech group who has supplied us with this part.
>>About pSB1 vs pSB6

NamePart TypeResistanceInsertVector
<partinfo>pSB6A1</partinfo>(<partinfo>BBa_K121013</partinfo>)Protein domainAmpicillin883bp4022bpRBS+GFP+T+T

We designed and constructed these parts originally.

We cloned the following inserts into the vector, pSB6A1. Out of them we cloned the two inserts(BBa_K362005, BBa_K362007) into the pSB1C3 to send to the iGEM headquarter.

<groupparts>iGEM010 KIT-Kyoto</groupparts>