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Contents 1 2010/10/03 1.1 1:PCR 1.2 2：DNA extraction 1.3 3:Electrophoresis 1.4 4：DNA extraction 1.5 5：DNA Ligation 1.6 6:Transformation 1.7 7：DNA Digestion

2010/10/03

1:PCR

<Member>
hito

<Sample>(temperature in annealing:℃)

• 1
• 2
• 3
• 4
• 5
• 6
• 7
• 8
• 9
• 10
• 1-2
• 4-5
• 7-8-10

<Protocol>
See Protocol 2

2：DNA extraction

＜Member＞
mio

＜Sample＞
PCR products

• 4②
• 4⑤

＜Protocol＞
See Protocol 4

3:Electrophoresis

<Member>
nito

<Sample>
All PCR products(10/3)

<Protocol>
See Protocol 8

<Result>

4：DNA extraction

＜Member＞
mio,mizuki,nito

＜Sample＞
PCR products

• 1
• 3
• 4
• 5
• 8
• 10
• 4-5
• 7-8-10
• 2
• 6

Digestion products

• 1
• 2
• 3
• 4
• 5
• 6
• 7
• 7-8-10

＜Protocol＞
See Protocol 4

5：DNA Ligation

＜Member＞
mio,mizuki

＜Sample＞

• 1-2
• 4-5
• 6A-7
• 7-8-10

(after digestion)

＜Protocol＞
See Protocol 10

6:Transformation

<Member>
nito

<Sample>

• 7-8-10
• JM109

<Protocol>
See Protocol 7

7：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

• 1
• 2
• 3
• 4
• 6
• 10

＜Protocol＞
See Prptpcpl 9