Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/17

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Contents

2010/09/17

1:PCR

<Member>
mio

<Sample>
(temperature in annealing)

<Protocol>
See Protocol 1

2：DNA Digestion

＜Member＞
mizuki

＜Sample, Materials＞
・PCR productions

  • 1 (8/31)
  • 2 (9/6)
  • 4 (8/31)
  • 5L (8/26)
  • 7 (8/31)
  • 8 (8/31)

・Digest enzyme

  • 1,4,8 : AvrⅡ
  • 2,7 : NheⅠ
  • 5L : SpeⅠ


＜Protocol＞
See Prptpcpl 9

3:Electrophoresis①

<Member>
mio

<Sample>
PCR products (9/17)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-17 17hr 10min.JPG

4:Electrophoresis②

<Member>
mizuki

<Sample>
Digestion products (9/17)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-17 18hr 05min.JPG

5：DNA extraction from gels

＜Member＞
mizuki

＜Sample＞
Digestion products(9/17)

(After electrophoresis)

＜Protocol＞
See Protocol 4

6：DNA Ligation

＜Member＞
mizuki

＜Sample＞

  • 7(9/17)
  • 8(9/17)

(after digestion)

＜Protocol＞
See Protocol 10

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/17"