[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Debrecen-Hungary Our parts]

Our lipid detecting tool kit comprosis of 19 basic parts (11 ligand binding domains, 3 DNA binding domains and 5 other basic parts),9 composite chimeric receptors and 3 plasmid expression vectors and associated intermediates. Amongst our kit, parts of various speicies of the animalia kingdom may be found. The composite receptors are proof of concept that the basic parts acctually work. The bulk of the data (sequance, sub domains, 3d structure, ligand affinity and more..) can be found in the parts registry.

Ligand binding domains - Basic Parts

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364310 Ecdysone Receptor Ligand Binding Domain (D. Melanogaster)]

The ecdysone receptor is a nuclear receptor found in arthropods, where it controls development and contributes to other processes such as reproduction. The receptor is a non-covalent heterodimer of two proteins, the EcR protein and ultraspiracle protein (USP). It binds to and is activated by ecdysteroids. Pulses of 20-hydroxyecdysone occur during insect development, whereupon this hormone binds to the ecdysone receptor, a ligand-activated transcription factor found in the nuclei of insect cells. This in turn leads to the activation of many other genes, which ultimately causes physiological changes that result in ecdysis (moulting). [http://partsregistry.org/wiki/index.php?title=Part:BBa_K364310 Go to the official parts registry page for this part]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364303 DAF 12 Nuclear Receptor(C. Elegans)]

The nuclear hormone receptor DAF-12 from Caenorhabditis elegans is activated by dafachronic acids, which derive from sterols upon oxidation by DAF-9, a cytochrome P450. DAF-12 activation is a critical checkpoint in C. elegans for acquisition of reproductive competence and for entry into adulthood rather than dauer diapause. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K364303 Go to the official parts registry page for this part]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364314 Nuclear hormone receptor 8 (C. Elegans)]

Nuclear hormone receptor family member nhr-8 The nhr-8 gene encodes a nuclear hormone receptor homolog; nhr-8(ok186) mutants have abnormally low resistance to the toxins colchicine and chloroquine. NHR-8 functions in the nematode xenobiotic defense system. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K364314 Go to the official parts registry page for this part]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364315 Nuclear hormone receptor 23 (C. Elegans)]

Nuclear hormone receptor family member nhr-23. The nhr-23 gene encodes a nuclear hormone receptor homolog that is required in all larval molts; NHR-23 is highly similar to Drosophila DHR3, an ecdysone-inducible gene product involved in metamorphosis. The NHR-23 protein is nuclear, and is present in all blastomeres during early embryogenesis; during later stages of morphogenesis, NHR-23 is restricted to epidermal cells. nhr-23 expression cycles between stages of larval development; during each intermolt period, levels of nhr-23 transcripts are 2-5 times greater than levels at each molt. NHR-23 binds the DRS-type hormone response sequence in vitro [http://partsregistry.org/wiki/index.php?title=Part:BBa_K364315 Go to the official parts registry page for this part]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364316 Nuclear hormone receptor 25 (C. Elegans)]

Nuclear hormone receptor family member nhr-25. nhr-25 encodes a nuclear hormone receptor orthologous to Drosophila Ftz-F1; NHR-25 is required for embryogenesis, molting, vulval and gonadal development, and hypodermal expression of acn-1; nhr-25 is expressed in gonads and loaded into embryos as a maternal transcript; nhr-25 is zygotically expressed in progeny of the E cell, and then in hypodermis and gut; the role of NHR-25 in molting may be evolutionarily conserved between nematodes and arthopods. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K364316 Go to the official parts registry page for this part]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364317 Nuclear hormone receptor 31 (C. Elegans)]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364317 Go to the official parts registry page for this part]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364321 Nuclear hormone receptor 64(C. Elegans)]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364321 Go to the official parts registry page for this part]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364318 Nuclear hormone receptor 80 (C. Elegans)]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364318 Go to the official parts registry page for this part]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364306 Constitutive Androstane Receptor Ligand Binding Domain (H. Sapiens)]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364306 Go to the official parts registry page for this part]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364308 Pregnane X receptor Ligand Binding Domain (H. Sapiens)]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364308 Go to the official parts registry page for this part]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364309 Estrogen Receptor Ligand Binding Domain (H. Sapiens)]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364309 Go to the official parts registry page for this part]

DNA binding domains - Basic Parts

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364319 Gal 4 DNA binding Domain (S. cerevisiae)]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364201 Retinoid X Receptor DNA binding Domain (H.Sapiens)]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364307 Estrogen Receptor DNA Binding Domain (H.Sapiens)]

Other Basic Parts

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364312 Vitamin D3 Receptor hinge region (H. Sapiens)]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364304 Tetracyclin responsive DNA element in a CMV protmoter (TRE-CMV)]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364302 Poly A tail type terminator]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364202 BCL-2 Associated X protein (H. Sapiens)]

Chimeric Nuclear Receptors - Composite Parts

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364328 Gal4-DAF12 Chimeric nuclear receptor]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364320 Gal4-NHR8 Chimeric nuclear receptor]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364322 Gal4-NHR23 Chimeric nuclear receptor]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364323 Gal4-NHR31 Chimeric nuclear receptor]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364324 Gal4-NHR80 Chimeric nuclear receptor]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364325 Gal4-PXR Chimeric nuclear receptor]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364326 Gal4-EcR Chimeric nuclear receptor]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364327 Gal4-ER Chimeric nuclear receptor]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364329 ER DBD-VDR hinge-ER LBD Chimeric nuclear receptor]

Eukaryotic expression vectors - Composite Parts

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364330 TRE-PolyA (intermediate part of the expression vector)]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364332 TRE-Gal4-PXR-PolyA in PSB1A3 (expression vector)]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K364333 TRE-Gal4-EcR-PolyA in PSB1A3 (expression vector)]