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Contents 1 2010/10/15 1.1 1：DNA extraction 1.2 2:Electrophoresis 1.3 3：DNA extraction from gels 1.4 4:PCR 1.5 5:Electrophoresis 1.6 6:PCR 1.7 7：DNA Digestion 1.8 8：DNA Ligation 1.9 9:PCR 1.10 10:PCR 1.11 11:Electrophoresis 1.12 12:Transformation 1.13 13:Electrophoresis 1.14 14:Electrophoresis

2010/10/15

1：DNA extraction

＜Member＞
Hitomi

＜Sample＞

• 4-5
• 9-7
• vector

＜Protocol＞
SeeProtocol 4

2:Electrophoresis

<Member>
Hitomi

<Sample>

• 4-5
• 9-7

<Protocol>
See Protocol 8

<Result>

3：DNA extraction from gels

＜Member＞
Mariko

＜Sample＞

• 4-5

(After electrophoresis)

＜Protocol＞
SeeProtocol 4

4:PCR

<Member>
Hitomi

<Sample>(temperature in annealing:℃)

• vector
• 4-5
• 9-7

<Protocol>
See Protocol 2

5:Electrophoresis

<Member>
nito

<Sample>

• 4-5
• 9-7
• 9
• vector

<Protocol>
See Protocol 8

<Result>

6:PCR

<Member>
mio

<Sample>

• 9

<Protocol>
See Protocol 2

7：DNA Digestion

＜Member＞
Hitomi

＜Sample, Materials＞

• vector
• 9-7

＜Protocol＞
See Protocol 9

8：DNA Ligation

＜Member＞
Hitomi

＜Sample＞

• 1-2-3
• 4-5
• 6-7
• 9-7

＜Protocol＞
See Protocol 3

9:PCR

<Member>
mio

<Sample>(temperature in annealing:℃)

• 9
• 9-7
• 1-2-3
• 4-5
• 6-7
• 9-7

<Protocol>
See Protocol 2

10:PCR

<Member>
nito,mizuki

<Sample>

• 9
• vector

<Protocol>
See Protocol 2

11:Electrophoresis

<Member>
mio

<Sample>

• [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9

<Protocol>
See Protocol 8

<Result>

12:Transformation

<Member>
nito

<Sample>

• 7-8-10
• 1-2-7
• 10-2-7
• JM109

<Protocol>
See Protocol 7

13:Electrophoresis

<Member>
nito

<Sample>

• 1-2-3
• 4-5
• 6-7
• 9-7
• 1

<Protocol>
See Protocol 8

<Result>

14:Electrophoresis

<Member>
nito

<Sample>

• 9
• vector

<Protocol>
See Protocol 8

<Result>