Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/15

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Contents

2010/10/15

1:DNA extraction

<Member>
Hitomi

<Sample>

<Protocol>
SeeProtocol 4


2:Electrophoresis

<Member>
Hitomi

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-15 12hr 41min.JPG


3:DNA extraction from gels

<Member>
Mariko

<Sample>

(After electrophoresis)

<Protocol>
SeeProtocol 4


4:PCR

<Member>
Hitomi

<Sample>(temperature in annealing:℃)

<Protocol>
See Protocol 2


5:Electrophoresis

<Member>
nito

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-15 19hr 19min.JPG


6:PCR

<Member>
mio

<Sample>

<Protocol>
See Protocol 2


7:DNA Digestion

<Member>
Hitomi

<Sample, Materials>

<Protocol>
See Protocol 9


8:DNA Ligation

<Member>
Hitomi

<Sample>

<Protocol>
See Protocol 3


9:PCR

<Member>
mio

<Sample>(temperature in annealing:℃)

<Protocol>
See Protocol 2


10:PCR

<Member>
nito,mizuki

<Sample>

  • 9
  • vector

<Protocol>
See Protocol 2

11:Electrophoresis

<Member>
mio

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-15 22hr 32min.JPG

12:Transformation

<Member>
nito

<Sample>

  • 7-8-10
  • 1-2-7
  • 10-2-7
  • JM109


<Protocol>
See Protocol 7


13:Electrophoresis

<Member>
nito

<Sample>

  • 1-2-3
  • 4-5
  • 6-7
  • 9-7
  • 1


<Protocol>
See Protocol 8


<Result>
Matsuura 2010-10-16 03hr 24min.JPG


14:Electrophoresis

<Member>
nito

<Sample>

  • 9
  • vector


<Protocol>
See Protocol 8


<Result>
Matsuura 2010-10-16 01hr 45min.JPG