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Contents 1 2010/10/10 1.1 1:Electrophoresis 1.2 2:PCR 1.3 3：DNA Digestion 1.4 4：DNA Ligation 1.5 5：DNA extraction 1.6 6:Plasmid extraction from E-coli

2010/10/10

1:Electrophoresis

<Member>
nito

<Sample>
PCR products (10/10)

<Protocol>
See Protocol 8

<Result>

<Result>

<Result>

<Result>
File:Image:Matsuura 2010-10-10 21hr 24min.JPG

2:PCR

<Member>
nito

<Sample>(temperature in annealing:℃)

• 9 (60)
• 1-2 (60)
• 2-10 (60)
• 7-8-10 (60)

<Protocol>
See Protocol 1

3：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

• 5

・Digest enzyme

• Spe1

＜Protocol＞
See Prptpcpl 9

4：DNA Ligation

＜Member＞
nito

＜Sample＞

• 1-2
• 10-2

(after digestion)

＜Protocol＞
See Protocol 10

5：DNA extraction

＜Member＞
Mariko,mio,nito

＜Sample＞
PCR products

• 2
• 4
• 8
• 10
• 5

＜Protocol＞
See Protocol 4

6:Plasmid extraction from E-coli

<Member>
nito

<Sample>

• 12
• 13
• 15
• 16

<Protocol>
See Protocol 6