"

From 2010.igem.org

Home

Team

Project

Parts

Notebook

Human Practice

Judging Criteria

Acknowledgment

Contents 1 2010/9/27 Monday (watachin) 1.1 PCR 1.2 Electrophoreses of PCR production 1.3 Digestion 1.4 Electrophoreses of PCR productions 1.5 DNA Purification 1.6 Ligation 1.7 DNA Purification 1.8 Ligation 1.9 Transfomation 1.10 PCR 1.11 Electrophoreses of PCR productions

2010/9/27 Monday (watachin)

PCR

PCR of A.xylinum bcsA,D and E.coli bcsA,B,C ,pSB1C3

Electrophoreses of PCR production

Result

Digestion

bcsA → Xba,Spe

bcsB → Xba

bcsC → Spe

pSB1C3 → Xba, Spe

Electrophoreses of PCR productions

Result

DNA Purification

Ligation

DNA Purification

Ligation

Transfomation

PCR

Electrophoreses of PCR productions

Result