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Contents 1 2010/10/06 Wednesday (Naoto) 1.1 Experiment:Electrophoresis of insert check PCR 1.2 Experiment:PCR of T7promoter~bcsABCD(A.xylinum),T7promoter~bcsEFG(E.coli),RBS~T7polymerase 1.3 Experiment:Measure DNA density

2010/10/06 Wednesday (Naoto)

Experiment:Electrophoresis of insert check PCR

member

naoto

material

• insert check PCR production(PCR at 10/05)

If you want to know other materials,see protocol4

procedure

see protocol4

result

All of bands seemed to be vector self ligation

Experiment:PCR of T7promoter~bcsABCD(A.xylinum),T7promoter~bcsEFG(E.coli),RBS~T7polymerase

member

naoto

material

• A colony of A.xylinum
• bcsEFG(E.coli)
• T7 polymerase(BBa_K145001)

If you want to know other materials,see protocol3

procedure

see protocol3

Experiment:Measure DNA density

member

naoto

material

• absorption spectrometer
• bcsA,B,C,D(A.xylinum)
• bcsA,B,C,EFG(E.coli)
• pSB1C3
• TE buffer

procedure

• mix 2µl of DNA solution with 2µl of TE buffer
• spot DNA solution and TE buffer mixture to absorption spectrometer
• measure DNA density