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Contents
October
October 2, 2010
ADS
Objective: Purify plasmid DNA from transformed Mr. GENE synthesized DNA
- <partinfo>K331022</partinfo>
- <partinfo>K331023</partinfo>
- <partinfo>K331024</partinfo>
- <partinfo>K331025</partinfo>
Method: Use Qiagen miniprep method with BioBasic EZ-10 Spin columns.
Objective: Create 20 new biobricks using recently arrived synthesized DNA and terminator <partinfo>B0015</partinfo>, pLac <partinfo>R0010</partinfo>, and pTet <partinfo>R0040</partinfo> to create the following new biobricks:
- 1)R0010 + K331022 to obtain <partinfo>K331026</partinfo>
- 2)R0040 + K331022 to obtain <partinfo>K331030</partinfo>
- 3)K331022 + B0015 to obtain <partinfo>K331034</partinfo>
- 4)K331022 + K331024 to obtain (NAME BIOBRICK!!!!)
- 5)R0010 + K331023 to obtain <partinfo>K331027</partinfo>
- 6)R0040 + K331023 to obtain <partinfo>K331031</partinfo>
- 7)K331023 + B0015 to obtain <partinfo>K331035</partinfo>
- 8)K331023 + K331025 to obtain (NAME BIOBRICK!!!!)
- 9)R0010 + K331024 to obtain <partinfo>K331028</partinfo>
- 10)R0040 + K331024 to obtain <partinfo>K331032</partinfo>
- 11)K331024 + B0015 to obtain <partinfo>K331036</partinfo>
- 12)K331024 + K331022 to obtain (NAME BIOBRICK!!!!)
- 13)R0010 + K331025 to obtain <partinfo>K331029</partinfo>
- 14)R0040 + K331025 to obtain <partinfo>K331033</partinfo>
- 15)K331025 + B0015 to obtain <partinfo>K331037</partinfo>
- 16)K331025 + K331023 to obtain (NAME BIOBRICK!!!!)
- 17)K331022 to obtain <partinfo>K331022</partinfo>
- 18)K331023 to obtain <partinfo>K331023</partinfo>
- 19)K331024 to obtain <partinfo>K331024</partinfo>
- 20)K331025 to obtain <partinfo>K331025</partinfo>
All parts will be inserted into pSB1C3.
Method: Use BioBrick Assembly Method
Results: Obtained RESULTS!!! positive colonies.October 5, 2010
ADS
Objective: Purify plasmid DNA from successfully assembled biobricks.
Method: Use Qiagen miniprep method with BioBasic EZ-10 columns.
Will send these minipreps for sequencing at Macrogen.