From 2010.igem.org

Metabolic Flux Analysis

A metabolic flux analysis is an analysis to calculate the theoretical maximal yields for a proposed system of pathways in a micro-organism. With this analysis it is also possible to find whether a system is not viable because of a co-factor imbalance. Lastly, several product pathways were introduced to E. coli with hydrocarbon degradation to see what maximal theoretical yields on hydrocarbons are.

How a metabolic flux analysis works, is explained here.

For our MFA a Matlab application, Cell Net Analyzer, was used. CNA already contains a standard simplified E. coli metabolic network. This network was slightly changed and hydrocarbon degradation was added to it. (link to pages with assumptions) The resulting network is the standard network used for adding additional pathways.

• implement interactive tool with metabolic network for different pathways here

Implemented pathways

• NO3 as electron acceptor
• PHB production
• isoprene production
• H2 production

refer to detailed results page here

Detailed MFA results

Go back to previous page here

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