Revision as of 10:08, 7 September 2010 by RachelBoyd (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Contents 1 yneA 1.1 Aims 1.2 Materials and protocols 1.3 Results 1.4 Conclusion 2 rocF 2.1 Aims 2.2 Materials and protocols 2.3 Results

yneA

Aims

Purify more yneA in pGFPrrnb and check with restriction enzymes.

Materials and protocols

Plasmid extraction Restriction digest Gel electrophoresis

Results

Conclusion

The results show that the digest works, there is a band at 541bp corresponding to yneA and a band at 8.4kbp corresponding to GFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12.

rocF

Aims

Materials and protocols

Plasmid extraction Nanodrop

Results

Nanodrop results

1. 264.4 ng/ul
2. 267.6 ng/ul
3. 309.5 ng/ul
4. 160.1 ng/ul pure ~ 1.87
5. 218.4 ng/ul
6. 233.4 ng/ul
7. 258.0 ng/ul
8. 201.7 ng/ul
9. 237.8 ng/ul
10. 201.7 ng/ul