Team:METU Turkey/Results Discussion/CooA Expression and Purification

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3-CooA Expression and Purification



1-Overexpression optimization
A. Expression vector comparison (pKK versus pTriEx)
A.1. Expression in BL21 with pTriEx

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A.2. Expression in JM109 and BL21 with pKK vector

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B. Expression host comparison (JM109 versus BL21)



C. IPTG induction studies


D. Ferric Citrate effect


E. Soluble versus insoluble fraction


2-Purification optimization


A. Ion exchange chromatography with Sepharose Q (QS)





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