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Team:Imperial College London/Modelling/Signalling/Results and Conclusion
From 2010.igem.org
| Modelling | Overview | Detection Model | Signaling Model | Fast Response Model | Interactions |
| A major part of the project consisted of modelling each module. This enabled us to decide which ideas we should implement. Look at the Fast Response page for a great example of how modelling has made a major impact on our design! | |
| Objectives | Description | Results | Constants | MATLAB Code |
| Results and Conclusion | ||
| Using this model, we can show that the phosphorylated ComE* is proportional to both initial concentration of AIP and ComD.
If the initial concentration of AIP or ComD is zero, there is no formation of ComE*. We are assuming an initial concentration of Phosphate and ComE of 100nM. If we change either [AIP]0 or [ComE]0, then the final concentration of ComE* will always tend towards 5×10-11M. [ComE*]final will always tend towards this value, unless the initial concentrations of Phosphate and ComE are changed. However, if we increase both [AIP]0 and [ComE]0 at the same time, then [ComE*]final will be reached much faster (i.e. slope increases). | ||
| Final Equation: AIP-ComD*-ComE ↔ AIP-ComD + ComE* | ||
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| Equation 1: AIP + ComD ↔ AIP-ComD | ||
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| Equation 2: AIP-ComD + Phosphate ↔ AIP-ComD* | ||
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| Equation 3: AIP-ComD* + ComE ↔ AIP-ComD*-ComE | ||
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