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Team:Newcastle/10 September 2010
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Contents |
Sending off yneA BioBrick
Aim
The aim of this experiment is to extract ligated yneA fragment in the plasmid pSB1C3 from E. coli cells and purify them and freeze dry them to send them off to the iGEM headquarters.
Materials and Protocol
Please refer to: Qiagen Minipreps for plasmid extraction from E. coli cells.
Please refer to: Nanodrop Spectrophotometer to check the purity of the extracted and purified plasmid.
Result
| Tube | Nanodrop Value (in ng/ml) |
|---|---|
| 1 | 158.7 |
| 2 | 261.2 |
| 3 | 262.9 |
| 4 | 204.7 |
| 5 | 262.9 |
| 6 | 202.1 |
| 7 | 174.8 |
| 8 | 226.6 |
| 9 | 229.1 |
| 10 | 164.5 |
| 11 | 177.8 |
| 12 | 225.1 |
| 13 | 281.5 |
| 14 | 249.3 |
| 15 | 192.2 |
| 16 | 239.3 |
   
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