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27 July 2010

Contents 1 Genomic DNA extraction experiment 1.1 Aims 1.2 Protocol 1.3 Discussion 1.4 Conclusion

Genomic DNA extraction experiment

Aims

The aim of today's experiment is to extract genomic DNA from both B. subtilis strains 168 and 3610. The genes necessary for the swarming BioBrick and rocF BioBrick will then hopefully be obtained from the genomic DNA using PCR.

Protocol

• Please refer to: DNA extraction of B. subtilis for materials required and protocol.

Discussion

At the end of the DNA precipitation step, we observed a small white pellet in all the eppendorf tubes.

Conclusion

The experiment was a success! The content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010.