"
Team:Newcastle/Meetings/21 October 2010
From 2010.igem.org
| |||||||||||||
| |||||||||||||
Apologies - Phil, Younus, Deena late
Action point: Phil and Rachel add characteristation data to the wiki
Contents |
yneA Characteristaion:
- ΔamyE:Pspac(hy)::yneA
- Bacillus subtilis spread of IPTG may not be homogenous through the population.
- E.coli has transporter deltions: check E.coli wiki
take out pMap65 results
Median, Mode add to graph
pMutin4 proportion of cells
20% responding- looks like
Biomass of cells - length * amount(counts)) against IPTG
Lysis acts to counter the overexpression of yneA ... so we have fewer cells
Younus AP: send script for yneA to Jen, Matt, Neil
Plot: Max cell length against ITPG + Error bars
Arabinose
We think we have it cloned infront of GFP Bacillus subtilis transformation tommorrow Fluorescence Characterisation of previous part!!!
Concrete
Damaged building- earthquake
Flux balance
difference between optimal and normal
Lab work
DNA submitted... tracking number sent 1C3 SR1 has not arrived, we would have had 2 bricks submitted!
Safety page
Deena and Harsh
Risk assessment- Bacillus
BioCOSH
Safety Guy talk mention it
Presentation
Phil DON'T BE AGGRESSIVE!!!!
Begining:
- say what the project is about
- mention Bacillus subtilis
- Why bacillus
- bioconcrete- show its current
Non-pathogenic not non-virulent Previous work on CaCO3 Don't use the word PROVES Separate slid for work flows
Alan- Flux first, speak slowly
Summary table- redo
BRONZE, SILVER, GOLD!!!!!!!
GOLD
Jannetta write RFC-
- number on judging form
- webservice!!!
SEM
Images are ours
Other
Insurance form Taxi
Younus Poster!!! next thursday due in?
Action point check presentation and poster submission!!!
