Home
People
Project Details
Protocols
Completed Systems
Notebook
Biosafety
Human Impact
References
Support
|
Back to Notebook
Monday 6/28
- Tested the success of the construct we made using digest and gel electrophoresis.
- Digested ligation product from 6/26 with EcoRI and PstI.
- Ran digests on gel along with 1kb and 100kb molecular ladders.
- Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.
 Image of a test gel of our third ligation product. Note that the middle two lanes are sadly empty. - Transformed additional BioBricks from 2010 Spring DNA Distribution into DH5alpha cells
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 K274002], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 K274003], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 K274004]
Tuesday 6/29
- Transformation results: Transformation of the ampicillin-resistant BioBricks seemed to be successful. However, no bacterial growth was present in the plates and liquid cultures with tetracyclin present. This seems to indicate a problem with the tetracyclin used.
- Of the plates with bacterial growth ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100 (red)], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200 (orange)]), there was evidence of dye production, indicating a successful transformation.
- Transformed 7 more bricks (pigments & our AND gate components):
Wednesday 6/30
Thursday 7/1
Friday 7/2
Weekend 7/3-4
|
"
