Team:Tsinghua/project/outline

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=Project Outline=
 
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Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology. The Aim is to Simulate Antibody Selection and Production Technology with Bacteria.
 
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Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody.
 
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In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:
 
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MODULE I: Generation of Antibody Library
 
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MODULE II: Selection of Specific Antibody
 

Revision as of 14:19, 12 October 2010

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Module I

TSModule2m.PNG

Abstract

Flow chart

Landing Pad Insertion

Helper Plasmid(HP) Insertion

Donor Plasmid(DP) Construction

Strategy 1

Strategy 2

Donor Plasmid(DP) Insertion & Recombination Induction

Removal of Helper Plasmid(HP)


Module II

Strategy 1

TSModule2m.PNG

Strategy 2

TSModule2s.PNG


Strategy 3

Cooperation with Macquarie Australia

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