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Team:Washington/Tools Created/New Vectors
From 2010.igem.org
(→Protein Expression Vectors) |
(→Protein Expression Vectors) |
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=='''Testing the Vectors'''== | =='''Testing the Vectors'''== | ||
==='''Protien Expression'''=== | ==='''Protien Expression'''=== | ||
Revision as of 22:02, 8 October 2010
Protein Expression Vectors
As part of the protein expression process in the gram positive theraputics portion
Vector Design
f1 origin
Lac I
Promoters
The expression vectors promoter are available in constitutive and inducible variety. The [http://partsregistry.org/Part:BBa_J23100 Constitutive promoters] are BBa J23100, J23113, and J23114. Inducible vectors include [http://partsregistry.org/Part:BBa_R0011 R0011] and T7, both of which are repressed by the Lac I protein.
Ribosome Binding Site
The Elowitz standard RBS [http://partsregistry.org/Part:BBa_B0034 B0034] is used on all vectors.
Building the Vectors
overview of what was done in one paragraph... links to protocols used (need to put up pcr (taq/fusion) digestion, ligation, transformation links to registry)
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Testing the Vectors
Protien Expression
The vector cassette was placed in 4 different plasmid backbone from the registry(psb1c3, psb1a3, psb3k3, psb4a5.. make into links) and GFP (BBa E0040) was placed in the protein expression area of the vector. Data was pulled and expressed below....
f1 origin
The f1 origin was tested by compairing infected verse none infected DNA isolation amounts (explain in more detail once this is done)
