Team:Washington/Tools Created/New Vectors

From 2010.igem.org

(Difference between revisions)
(→Protein Expression Vectors)
Line 51: Line 51:
overview of what was done in one paragraph...
overview of what was done in one paragraph...
links to protocols used (need to put up pcr (taq/fusion) digestion, ligation, transformation links to registry)
links to protocols used (need to put up pcr (taq/fusion) digestion, ligation, transformation links to registry)
 +
 +
[[Image:uw_vectors_button.jpg|150px|left]]
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
<br />
 +
<br />
 +
 +
[[Image:uw_promoters_button2.jpg|140px|left]]
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
<br />
 +
<br />
 +
[[Image:uw_Lac_button.jpg|140px|left]]
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
<br />
 +
<br />
 +
[[Image:uw_AB_button.jpg|140px|left]]
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blahblah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blahblah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blahblah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
[[Image:uw_RBS_button.jpg|140px|left]]
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blahblah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blahblah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
[[Image:uw_F1_button.jpg|140px|left]]
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blahblah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blahblah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
[[Image:uw_copy_button.jpg|140px|left]]
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blahblah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blahblah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
[[Image:uw_cut_button.jpg|100px|left]]
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blahblah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blahblah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
blah blah blah blah
 +
<br />
 +
<br />
 +
<br />
 +
<br />
 +
<br />
 +
<br />
<br />
<br />
<br />
<br />

Revision as of 22:01, 8 October 2010

Washington 2010 vector logo2.jpg

Protein Expression Vectors

As part of the protein expression process in the gram positive theraputics portion

Vector Design

Vector
Vector


f1 origin

Lac I

Promoters

The expression vectors promoter are available in constitutive and inducible variety. The [http://partsregistry.org/Part:BBa_J23100 Constitutive promoters] are BBa J23100, J23113, and J23114. Inducible vectors include [http://partsregistry.org/Part:BBa_R0011 R0011] and T7, both of which are repressed by the Lac I protein.

Ribosome Binding Site

The Elowitz standard RBS [http://partsregistry.org/Part:BBa_B0034 B0034] is used on all vectors.

Building the Vectors

overview of what was done in one paragraph... links to protocols used (need to put up pcr (taq/fusion) digestion, ligation, transformation links to registry)

Uw vectors button.jpg

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah



Uw promoters button2.jpg

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah



Uw Lac button.jpg

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

Uw AB button.jpg

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blahblah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blahblah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blahblah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

Uw RBS button.jpg

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blahblah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blahblah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

Uw F1 button.jpg

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blahblah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blahblah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

Uw copy button.jpg

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blahblah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blahblah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

Uw cut button.jpg

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blahblah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blahblah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah

blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah blah



















Testing the Vectors

Protien Expression

The vector cassette was placed in 4 different plasmid backbone from the registry(psb1c3, psb1a3, psb3k3, psb4a5.. make into links) and GFP (BBa E0040) was placed in the protein expression area of the vector. Data was pulled and expressed below....

Placeholder...

f1 origin

The f1 origin was tested by compairing infected verse none infected DNA isolation amounts (explain in more detail once this is done)

Placeholder...


← Next-Gen Cloning       Safety Information →

Retrieved from "http://2010.igem.org/Team:Washington/Tools_Created/New_Vectors"