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Revision as of 15:04, 3 October 2010 (view source) Naoto (Talk | contribs) (New page: {{:Team:Tokyo_Metropolitan/Header}} ==2010/09/29 Wednesday (Naoto)== ===Experiment:Electrophoresis of insert check PCR=== '''member''' naoto and bambi75 '''material''' *PCR production ...) ← Older edit		Revision as of 15:36, 3 October 2010 (view source) Naoto (Talk | contribs) Newer edit →
Line 2:		Line 2:
	==2010/09/29 Wednesday (Naoto)==		==2010/09/29 Wednesday (Naoto)==
-	===Experiment:Electrophoresis of insert check PCR===	+	===Experiment:Insert check PCR===
	'''member'''		'''member'''
-	naoto and bambi75	+	naoto and NEX
	'''material'''		'''material'''
-	*PCR production (bcsD(''A.xylinum'') )	+	*bcsC,D(''A.xylinum'') in colonies of '''E.coli'''
-	If you want to know other materials,refer to protocol4	+	If you want to know other materials,refer to protocol3
	'''procedure'''		'''procedure'''
-	see protocol4	+	see protocol3
-		+
-	'''result'''	+
-	Bands didn't appear at all	+
-	===Experiment:Electrophoresis of bcsEFG,Q===	+	===Experiment:PCR of PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(E.coli),bcsQ(''E.coli''),RBS-T7polymerase===
	'''member'''		'''member'''
-	naoto and bambi75	+	naoto and NEX
	'''material'''		'''material'''
-	*PCR production (bcsEFG,Q(''E.coli'') )	+	*A colony of ''A.xylinum''
-	If you want to know other materials,refer to protocol4	+	*bcsE~G(E.coli)
		+	*bcsQ(''E.coli'')
		+	*T7polymerase(BBa_K145001)
		+	If you want to know other materials,refer to protocol3
	'''procedure'''		'''procedure'''
-	see protocol4	+	see protocol3
-	'''result'''	+	===Experiment:digestion of bcsB(''E.coli'')===
-	Although many bands appeared,bands of bcsEFG and bcsQ also appeared	+
-		+
-	===Experiment:Electrophoresis of insert check PCR===	+
	'''member'''		'''member'''
-	naoto and bambi75	+	naoto and easily
	'''material'''		'''material'''
-	*PCR production (bcsD(''A.xylinum'') )	+	*bcsB(''E.coli'')
-	If you want to know other materials,refer to protocol4	+	If you want to know other materials,refer to protocol6
	'''procedure'''		'''procedure'''
-	see protocol4	+	follow to protocol6
-	===Experiment:Purification bcsA(''E.coli'')===	+	===Experiment:subculture of ''A.xylinum''===
	'''member'''		'''member'''
-	naoto and bambi75	+	naoto and easily
	'''material'''		'''material'''
-	*bcsQ,EFG(''E.coli'') in agarose gel	+	see protocol1
-	If you want to know other materials,refer to protocol5	+	A.xylinum(subcultured at 9/1)
	'''procedure'''		'''procedure'''
-	follow to protocol5	+	see protocol1
-	===Experiment:digestion of bcsEFG,Q(''E.coli'')===	+	===Experiment:Transformation===
	'''member'''		'''member'''
-	naoto and bambi75	+	naoto and easily
	'''material'''		'''material'''
-	*bcsQ,EFG(''E.coli'')	+	*parts of bcsA,B(''A.xylinum'')
-	If you want to know other materials,refer to protocol6	+	If you want to know other materials,refer to protocol8
	'''procedure'''		'''procedure'''
-	follow to protocol6	+	see protocol8
-	===Experiment:Electrophoresis of bcsEFG,Q===	+	===Experiment:Electrophoresis of insert check PCR===
	'''member'''		'''member'''
-	naoto and bambi75	+	naoto
	'''material'''		'''material'''
-	*PCR production (bcsEFG,Q(''E.coli'') )	+	*PCR production (bcsC,D(''A.xylinum'') )
	If you want to know other materials,refer to protocol4		If you want to know other materials,refer to protocol4
Line 93:		Line 90:
	'''result'''		'''result'''
-	Both bands of bcsEFG and bcsQ disappeared
-	===Experiment:Transformation===	+	Bands(102 and 104) of bcsD(''A.xylinum'') could be seen slightly
		+
		+	So we prepared precultures for miniprep
		+
		+	===Experiment:Electrophoresis of PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(E.coli)===
	'''member'''		'''member'''
-	naoto and bambi75	+	naoto
	'''material'''		'''material'''
-	*parts of bcsA,B,C,D(''A.xylinum'') and bcsA(''E.coli'')	+	*PCR production (PT7-RBS-bcsA~D(''A.xylinum''),PT7-RBS-E~G(E.coli))
-	If you want to know other materials,refer to protocol8	+	If you want to know other materials,refer to protocol4
	'''procedure'''		'''procedure'''
-	see protocol8	+	see protocol4
		+
		+	'''result'''
		+
		+	All bands differed from collect length

---

Revision as of 15:36, 3 October 2010

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Contents 1 2010/09/29 Wednesday (Naoto) 1.1 Experiment:Insert check PCR 1.2 Experiment:PCR of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli),bcsQ(E.coli),RBS-T7polymerase 1.3 Experiment:digestion of bcsB(E.coli) 1.4 Experiment:subculture of A.xylinum 1.5 Experiment:Transformation 1.6 Experiment:Electrophoresis of insert check PCR 1.7 Experiment:Electrophoresis of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli)

2010/09/29 Wednesday (Naoto)

Experiment:Insert check PCR

member

naoto and NEX

material

• bcsC,D(A.xylinum) in colonies of E.coli

If you want to know other materials,refer to protocol3

procedure

see protocol3

Experiment:PCR of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli),bcsQ(E.coli),RBS-T7polymerase

member

naoto and NEX

material

• A colony of A.xylinum
• bcsE~G(E.coli)
• bcsQ(E.coli)
• T7polymerase(BBa_K145001)

If you want to know other materials,refer to protocol3

procedure

see protocol3

Experiment:digestion of bcsB(E.coli)

member

naoto and easily

material

• bcsB(E.coli)

If you want to know other materials,refer to protocol6

procedure

follow to protocol6

Experiment:subculture of A.xylinum

member

naoto and easily

material

see protocol1 A.xylinum(subcultured at 9/1)

procedure

see protocol1

Experiment:Transformation

member

naoto and easily

material

• parts of bcsA,B(A.xylinum)

If you want to know other materials,refer to protocol8

procedure

see protocol8

Experiment:Electrophoresis of insert check PCR

member

naoto

material

• PCR production (bcsC,D(A.xylinum) )

If you want to know other materials,refer to protocol4

procedure

see protocol4

result

Bands(102 and 104) of bcsD(A.xylinum) could be seen slightly

So we prepared precultures for miniprep

Experiment:Electrophoresis of PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli)

member

naoto

material

• PCR production (PT7-RBS-bcsA~D(A.xylinum),PT7-RBS-E~G(E.coli))

If you want to know other materials,refer to protocol4

procedure

see protocol4

result

All bands differed from collect length