Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/29

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Contents

2010/09/29 Wednesday (Naoto)

Experiment:Electrophoresis of insert check PCR

member

naoto and bambi75

material

  • PCR production (bcsD(A.xylinum) )

If you want to know other materials,refer to protocol4

procedure

see protocol4

result Bands didn't appear at all

Experiment:Electrophoresis of bcsEFG,Q

member

naoto and bambi75

material

  • PCR production (bcsEFG,Q(E.coli) )

If you want to know other materials,refer to protocol4

procedure

see protocol4

result Although many bands appeared,bands of bcsEFG and bcsQ also appeared

Experiment:Electrophoresis of insert check PCR

member

naoto and bambi75

material

  • PCR production (bcsD(A.xylinum) )

If you want to know other materials,refer to protocol4

procedure

see protocol4

Experiment:Purification bcsA(E.coli)

member

naoto and bambi75

material

  • bcsQ,EFG(E.coli) in agarose gel

If you want to know other materials,refer to protocol5

procedure

follow to protocol5

Experiment:digestion of bcsEFG,Q(E.coli)

member

naoto and bambi75

material

  • bcsQ,EFG(E.coli)

If you want to know other materials,refer to protocol6

procedure

follow to protocol6

Experiment:Electrophoresis of bcsEFG,Q

member

naoto and bambi75

material

  • PCR production (bcsEFG,Q(E.coli) )

If you want to know other materials,refer to protocol4

procedure

see protocol4

result Both bands of bcsEFG and bcsQ disappeared

Experiment:Transformation

member

naoto and bambi75

material

  • parts of bcsA,B,C,D(A.xylinum) and bcsA(E.coli)

If you want to know other materials,refer to protocol8

procedure

see protocol8