Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/17

From 2010.igem.org

(Difference between revisions)
(→4:Electrophoresis②)
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{{:Team:Tokyo_Metropolitan/Header}}
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=2010/09/17=
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===1:PCR===
===1:PCR===
<Member><br />
<Member><br />
mio
mio
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<Sample><br />(temperature in annealing)
<Sample><br />(temperature in annealing)
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*6
*6
*8
*8
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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
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＜Member＞<br />
＜Member＞<br />
mizuki
mizuki
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＜Sample, Materials＞<br />
＜Sample, Materials＞<br />
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＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
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<Member><br />
<Member><br />
mio
mio
-
 
<Sample><br />
<Sample><br />
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*6
*6
*8
*8
-
 
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
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<Result><br />
<Result><br />
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<Member><br />
<Member><br />
mizuki
mizuki
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<Sample><br />
<Sample><br />
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*7
*7
*8
*8
-
 
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
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<Result><br />
<Result><br />
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＜Member＞<br />
＜Member＞<br />
mizuki
mizuki
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＜Sample＞<br />
＜Sample＞<br />
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*8
*8
(After electrophoresis)
(After electrophoresis)
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＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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＜Member＞<br />
＜Member＞<br />
mizuki
mizuki
-
 
＜Sample＞<br />
＜Sample＞<br />
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*8(9/17)
*8(9/17)
(after digestion)
(after digestion)
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＜Protocol＞<br />
＜Protocol＞<br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]

Revision as of 13:25, 29 September 2010


Contents

2010/09/17

1:PCR

<Member>
mio

<Sample>
(temperature in annealing)

  • 3
  • 6
  • 8

<Protocol>
See Protocol 1


2：DNA Digestion

＜Member＞
mizuki

＜Sample, Materials＞
・PCR productions

  • 1 (8/31)
  • 2 (9/6)
  • 4 (8/31)
  • 5L (8/26)
  • 7 (8/31)
  • 8 (8/31)

・Digest enzyme

  • 1,4,8 : AvrⅡ
  • 2,7 : NheⅠ
  • 5L : SpeⅠ


＜Protocol＞
See Prptpcpl 9


3:Electrophoresis①

<Member>
mio

<Sample>
PCR products (9/17)

  • 3
  • 6
  • 8

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-17 17hr 10min.JPG

4:Electrophoresis②

<Member>
mizuki

<Sample>
Digestion products (9/17)

  • 1
  • 2
  • 4
  • 5
  • 7
  • 8

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-09-17 18hr 05min.JPG

5：DNA extraction from gels

＜Member＞
mizuki

＜Sample＞
Digestion products(9/17)

  • 4
  • 7
  • 8

(After electrophoresis)

＜Protocol＞
See Protocol 4


6：DNA Ligation

＜Member＞
mizuki

＜Sample＞

  • 7(9/17)
  • 8(9/17)

(after digestion)

＜Protocol＞
See Protocol 10

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/17"