Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/08
From 2010.igem.org
(Difference between revisions)
Line 17: | Line 17: | ||
*Tube(temperature in annealing) | *Tube(temperature in annealing) | ||
1.(71.0,70.5,70.0℃)<br /> | 1.(71.0,70.5,70.0℃)<br /> | ||
- | 2.(72.0,71.5℃) | + | 2.(72.0,71.5℃)<br /> |
- | 3.(70.0℃) | + | 3.(70.0℃)<br /> |
4.(70.0℃) | 4.(70.0℃) | ||
Revision as of 13:20, 29 September 2010
2010/09/08
1:PCR
<Member>
Hitomi
<Sample><brt />
- 5
- 1+2(ligation 2010/08/31)
- 4+5(ligation 2010/08/31)
- 7+8(ligation 2010/09/06)
<Protocol>
See Protocol 2
- Tube(temperature in annealing)
1.(71.0,70.5,70.0℃)
2.(72.0,71.5℃)
3.(70.0℃)
4.(70.0℃)
- Total 12 tubes.
2:Electrophoresis
<Member>
mizuki, Hitomi
<Sample>
PCR products.
- 5H
- 5M
- 5L
- 1-2H
- 1-2L
- 4-5
- 7-8
<Protocol>
See Protocol 8