Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/08

From 2010.igem.org

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*Tube(temperature in annealing)
*Tube(temperature in annealing)
1.(71.0,70.5,70.0℃)<br />
1.(71.0,70.5,70.0℃)<br />
-
2.(72.0,71.5℃)
+
2.(72.0,71.5℃)<br />
-
3.(70.0℃)
+
3.(70.0℃)<br />
4.(70.0℃)
4.(70.0℃)

Revision as of 13:20, 29 September 2010


2010/09/08

1:PCR

<Member>
Hitomi

<Sample><brt />

  • 5
  • 1+2(ligation 2010/08/31)
  • 4+5(ligation 2010/08/31)
  • 7+8(ligation 2010/09/06)

<Protocol>
See Protocol 2

  • Tube(temperature in annealing)

1.(71.0,70.5,70.0℃)
2.(72.0,71.5℃)
3.(70.0℃)
4.(70.0℃)

Total 12 tubes.

2:Electrophoresis

<Member>
mizuki, Hitomi

<Sample>
PCR products.

  • 5H
  • 5M
  • 5L
  • 1-2H
  • 1-2L
  • 4-5
  • 7-8

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-08-06 20hr 02min (17).JPG