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Team:Washington/Tools Created/New Vectors
From 2010.igem.org
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| + | ='''Protien Expression Vectors'''= | ||
| + | As part of the protien expression process in the gram positive theraputics portion | ||
| + | |||
| + | =='''Vector Design'''== | ||
| + | [[Image:Washington 2010 vector.jpg|Right|400px|Vector]] | ||
| + | ==='''f1 origin'''=== | ||
| + | |||
| + | ==='''Lac I'''=== | ||
| + | |||
| + | ==='''Promoters'''=== | ||
| + | link to the 4 BBa parts | ||
| + | |||
| + | ==='''Ribosome Binding Site'''=== | ||
| + | |||
| + | link to bba b0034 | ||
| + | |||
| + | =='''Building the Vectors'''== | ||
| + | overview of what was done in one paragraph... | ||
| + | links to protocols used (need to put up pcr (taq/fusion) digestion, ligation, transformation links to registry) | ||
| + | |||
| + | |||
| + | =='''Testing the Vectors'''== | ||
| + | ==='''Protien Expression'''=== | ||
| + | The vector cassette was placed in 4 different plasmid backbone from the registry(psb1c3, psb1a3, psb3k3, psb4a5.. make into links) and GFP (BBa E0040) was placed in the protein expression area of the vector. Data was pulled and expressed below.... | ||
| + | [[Image:Washington_CapD_Header_Picture.jpg|thumb|center|300px| Placeholder...]] | ||
| + | |||
| + | ==='''f1 origin'''=== | ||
| + | The f1 origin was tested by compairing infected verse none infected DNA isolation amounts (explain in more detail once this is done) | ||
| + | [[Image:Washington_CapD_Header_Picture.jpg|thumb|center|300px| Placeholder...]] | ||
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Revision as of 20:52, 26 September 2010
Protien Expression Vectors
As part of the protien expression process in the gram positive theraputics portion
Vector Design
f1 origin
Lac I
Promoters
link to the 4 BBa parts
Ribosome Binding Site
link to bba b0034
Building the Vectors
overview of what was done in one paragraph... links to protocols used (need to put up pcr (taq/fusion) digestion, ligation, transformation links to registry)
Testing the Vectors
Protien Expression
The vector cassette was placed in 4 different plasmid backbone from the registry(psb1c3, psb1a3, psb3k3, psb4a5.. make into links) and GFP (BBa E0040) was placed in the protein expression area of the vector. Data was pulled and expressed below....
f1 origin
The f1 origin was tested by compairing infected verse none infected DNA isolation amounts (explain in more detail once this is done)
