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Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/09
From 2010.igem.org
(Difference between revisions)
(New page: {{:Team:Tokyo_Metropolitan/Header}} ==2010/09/09 Thursday (Naoto)== ===Experiment:Ligation=== '''member''' naoto and NEX '''material''' *bcsA,B(from ''A.xylinus'') 10µl each *bcsC,D(fr...) |
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'''material''' | '''material''' | ||
| - | *bcsA,B(from ''A. | + | *bcsA,B(from ''A.xylinum'') 10µl each |
| - | *bcsC,D(from ''A. | + | *bcsC,D(from ''A.xylinum'') 10µl each |
*bcsA,B(from ''E.coli'') 10µl each | *bcsA,B(from ''E.coli'') 10µl each | ||
*ligation Mix(nippon gene) 20µl each | *ligation Mix(nippon gene) 20µl each | ||
Revision as of 15:23, 16 September 2010
2010/09/09 Thursday (Naoto)
Experiment:Ligation
member
naoto and NEX
material
- bcsA,B(from A.xylinum) 10µl each
- bcsC,D(from A.xylinum) 10µl each
- bcsA,B(from E.coli) 10µl each
- ligation Mix(nippon gene) 20µl each
procedure
- add material to PCR tube
- incubate (20℃ 10min)
Experiment:Electrophoresis
member
naoto and NEX
material
- ligation products(see above)
we show other material at protocol4
procedure
follow to protocol4
