Team:Calgary/29 August 2010
From 2010.igem.org
(Difference between revisions)
Emily Hicks (Talk | contribs) |
|||
Line 14: | Line 14: | ||
Today, I ran 1.0% gels of the enormous colony PCR that was done yesterday. The gels themselves can be seen to the right with labels. As well, I made overnight cultures of I0500-I13507 construction which will be plasmid prepped tomorrow. Finally, I helped Emily digest and phosphatase MalE so it could be inserted into the AK Biobrick vector. | Today, I ran 1.0% gels of the enormous colony PCR that was done yesterday. The gels themselves can be seen to the right with labels. As well, I made overnight cultures of I0500-I13507 construction which will be plasmid prepped tomorrow. Finally, I helped Emily digest and phosphatase MalE so it could be inserted into the AK Biobrick vector. | ||
+ | |||
+ | |||
+ | <u>Emily</u> | ||
+ | |||
+ | Today I made gels for Chris for his giant colony PCR. I also set up a digest of the PCR Purified malE and malE31 as well as the psB1AK3 vector with a combination of different restriction enzymes. Chris phosphotased the vectors and I will ligate and then transform and plate them tomorrow. | ||
}} | }} |
Revision as of 18:45, 30 August 2010
Sunday August 29, 2010
Weekends rock!!
Chris
Today, I ran 1.0% gels of the enormous colony PCR that was done yesterday. The gels themselves can be seen to the right with labels. As well, I made overnight cultures of I0500-I13507 construction which will be plasmid prepped tomorrow. Finally, I helped Emily digest and phosphatase MalE so it could be inserted into the AK Biobrick vector.
Emily
Today I made gels for Chris for his giant colony PCR. I also set up a digest of the PCR Purified malE and malE31 as well as the psB1AK3 vector with a combination of different restriction enzymes. Chris phosphotased the vectors and I will ligate and then transform and plate them tomorrow.