Team:Newcastle/24 August 2010

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The results from the nanodrop:
The results from the nanodrop:
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{|border=1
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|-
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!DNA
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!Concentration (ng/µl)
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|-
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!1
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!286.1
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|-
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!2
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!304.0
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|-
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!3
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!316.3
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|-
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!4
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!421.6
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|-
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!5
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!518.7
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|-
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!6
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!460.1
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|-
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!7
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!370.1
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|-
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!8
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!377.3
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|-
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!9
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!346.0
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|-
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!10
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!347.4
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|-
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!11
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!202.8
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|-
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!12
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!307.4
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|}
==Discussion==
==Discussion==

Revision as of 14:22, 24 August 2010

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Contents

Miniprep for pGFPrrnB with yneA

Aims

To produce more stocks of vector pGFPrrnB with insert yneA.

Materials and Protocol

Please refer to qiagen minipreps and nanodrop spectrophotometer for all 12 tubes of miniprep that we did.

Results

The results from the nanodrop:

DNA Concentration (ng/µl)
1 286.1
2 304.0
3 316.3
4 421.6
5 518.7
6 460.1
7 370.1
8 377.3
9 346.0
10 347.4
11 202.8
12 307.4

Discussion

The results from the nanodrop showed that we have produced high concentration of vector pGFPrrnB with insert yneA. We will then proceed to digestion.

Single and Double Digestion of pGFPrrnB with yneA

Aims

To check if the insert yneA has been inserted into vector pGFPrrnB.

Materials and Protocol

We are doing two digests for pGFPrrnB and yneA:

  • Single digest with HinDIII;
  • Double digest with EcoRI and Nhe1.


Please refer to restriction digests and gel electrophoresis.

Results

Gel electrophoresis results for digestion:

Discussion and Conclusion

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