Team:Calgary/22 August 2010
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Today, I put digests of CpxP to insert into the AK plasmid. Emily and I also spent time defrosting the freezer. I did plasmid preparations of NLPE, Controls and MalE with Alex. Finally, I did ligations of CpxP into pSB1AK3, MalE31 into pSB2K3, and ibpAB into pSB1AK3. The plates will be seen tomorrow. | Today, I put digests of CpxP to insert into the AK plasmid. Emily and I also spent time defrosting the freezer. I did plasmid preparations of NLPE, Controls and MalE with Alex. Finally, I did ligations of CpxP into pSB1AK3, MalE31 into pSB2K3, and ibpAB into pSB1AK3. The plates will be seen tomorrow. | ||
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+ | <u>Emily</u> | ||
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+ | Today I ran gels of the two remaining colony PCR's from yesterday: one of the putitive I0500-B0034-malE31-cpxR-I13507 circuit as well as the IO500-B0034-malE31 circuit in cpxR competant cells. | ||
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Latest revision as of 05:02, 24 August 2010
![University of Calgary iGEM Team 2010](http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Header4.png)
Sunday August 22, 2010
Weekends Rule!!
Chris
Today, I put digests of CpxP to insert into the AK plasmid. Emily and I also spent time defrosting the freezer. I did plasmid preparations of NLPE, Controls and MalE with Alex. Finally, I did ligations of CpxP into pSB1AK3, MalE31 into pSB2K3, and ibpAB into pSB1AK3. The plates will be seen tomorrow.
Emily
Today I ran gels of the two remaining colony PCR's from yesterday: one of the putitive I0500-B0034-malE31-cpxR-I13507 circuit as well as the IO500-B0034-malE31 circuit in cpxR competant cells.