11 August 2010

From 2010.igem.org

(Difference between revisions)
Line 20: Line 20:
[Details of Experiment]<BR>
[Details of Experiment]<BR>
(1)We made 1% Agarose Gel by using Agarose 0.2g and TEA 20μL.<BR>
(1)We made 1% Agarose Gel by using Agarose 0.2g and TEA 20μL.<BR>
-
(2)We mixed DNA 5μL, Marker 1μL and Loading Buffer 1μL.We applied each 5μL of them to gel.We applied Marker 5μL to the far left lane.<BR>
+
(2)We mixed DNA 5μL, Marker 1μL and Loading Buffer 1μL.<br>
 +
We applied each 5μL of them to gel.We applied Marker 5μL to the far left lane.<BR>
(3) 100V, 30min electrophoresed<BR>
(3) 100V, 30min electrophoresed<BR>
(4)Gel soaked in EtBr for 20min.<BR>
(4)Gel soaked in EtBr for 20min.<BR>

Revision as of 01:45, 21 August 2010

Home Team Project Parts Modeling Notebook Link Sponsors
July
MTWTFSS
      [http://2010.igem.org/wiki/index.php?title=1_July_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=2_July_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=3_July_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=4_July_2010&action=edit 4]
[http://2010.igem.org/wiki/index.php?title=5_July_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=6_July_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=7_July_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=8_July_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=9_July_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=10_July_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=11_July_2010&action=edit 11]
[http://2010.igem.org/wiki/index.php?title=12_July_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=13_July_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=14_July_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=15_July_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=16_July_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=17_July_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=18_July_2010&action=edit 18]
[http://2010.igem.org/wiki/index.php?title=19_July_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=20_July_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=21_July_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=22_July_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=23_July_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=24_July_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=25_July_2010&action=edit 25]
[http://2010.igem.org/wiki/index.php?title=26_July_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=27_July_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=28_July_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=29_July_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=30_July_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=31_July_2010&action=edit 31]
August
MTWTFSS
            [http://2010.igem.org/wiki/index.php?title=1_August_2010&action=edit 1]
[http://2010.igem.org/wiki/index.php?title=2_August_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=3_August_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=4_August_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=5_August_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=6_August_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=7_August_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=8_August_2010&action=edit 8]
[http://2010.igem.org/wiki/index.php?title=9_August_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=10_August_2010&action=edit 10] [http://2010.igem.org/11_August_2010 11] [http://2010.igem.org/wiki/index.php?title=12_August_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=13_August_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=14_August_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=15_August_2010&action=edit 15]
[http://2010.igem.org/wiki/index.php?title=16_August_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=17_August_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=18_August_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=19_August_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=20_August_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=21_August_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=22_August_2010&action=edit 22]
[http://2010.igem.org/wiki/index.php?title=23_August_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=24_August_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=25_August_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=26_August_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=27_August_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=28_August_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=29_August_2010&action=edit 29]
[http://2010.igem.org/wiki/index.php?title=30_August_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=31_August_2010&action=edit 31]
September
MTWTFSS
    [http://2010.igem.org/wiki/index.php?title=1_September_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=2_September_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=3_September_2010&action=edit 3] [http://2010.igem.org/wiki/index.php?title=4_September_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=5_September_2010&action=edit 5]
[http://2010.igem.org/wiki/index.php?title=6_September_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=7_September_2010&action=edit 7] [http://2010.igem.org/wiki/index.php?title=8_September_2010&action=edit 8] [http://2010.igem.org/wiki/index.php?title=9_September_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=10_September_2010&action=edit 10] [http://2010.igem.org/wiki/index.php?title=11_September_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=12_September_2010&action=edit 12]
[http://2010.igem.org/wiki/index.php?title=13_September_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=14_September_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=15_September_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=16_September_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=17_September_2010&action=edit 17] [http://2010.igem.org/wiki/index.php?title=18_September_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=19_September_2010&action=edit 19]
[http://2010.igem.org/wiki/index.php?title=20_September_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=21_September_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=22_September_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=23_September_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=24_September_2010&action=edit 24] [http://2010.igem.org/wiki/index.php?title=25_September_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=26_September_2010&action=edit 26]
[http://2010.igem.org/wiki/index.php?title=27_September_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=28_September_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=29_September_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=30_September_2010&action=edit 30]
October
MTWTFSS
        [http://2010.igem.org/wiki/index.php?title=1_October_2010&action=edit 1] [http://2010.igem.org/wiki/index.php?title=2_October_2010&action=edit 2] [http://2010.igem.org/wiki/index.php?title=3_October_2010&action=edit 3]
[http://2010.igem.org/wiki/index.php?title=4_October_2010&action=edit 4] [http://2010.igem.org/wiki/index.php?title=5_October_2010&action=edit 5] [http://2010.igem.org/wiki/index.php?title=6_October_2010&action=edit 6] [http://2010.igem.org/wiki/index.php?title=7_October_2010&action=edit 7] [http://2010.igem.org/8_October_2010 8] [http://2010.igem.org/wiki/index.php?title=9_October_2010&action=edit 9] [http://2010.igem.org/wiki/index.php?title=10_October_2010&action=edit 10]
[http://2010.igem.org/wiki/index.php?title=11_October_2010&action=edit 11] [http://2010.igem.org/wiki/index.php?title=12_October_2010&action=edit 12] [http://2010.igem.org/wiki/index.php?title=13_October_2010&action=edit 13] [http://2010.igem.org/wiki/index.php?title=14_October_2010&action=edit 14] [http://2010.igem.org/wiki/index.php?title=15_October_2010&action=edit 15] [http://2010.igem.org/wiki/index.php?title=16_October_2010&action=edit 16] [http://2010.igem.org/wiki/index.php?title=17_October_2010&action=edit 17]
[http://2010.igem.org/wiki/index.php?title=18_October_2010&action=edit 18] [http://2010.igem.org/wiki/index.php?title=19_October_2010&action=edit 19] [http://2010.igem.org/wiki/index.php?title=20_October_2010&action=edit 20] [http://2010.igem.org/wiki/index.php?title=21_October_2010&action=edit 21] [http://2010.igem.org/wiki/index.php?title=22_October_2010&action=edit 22] [http://2010.igem.org/wiki/index.php?title=23_October_2010&action=edit 23] [http://2010.igem.org/wiki/index.php?title=24_October_2010&action=edit 24]
[http://2010.igem.org/wiki/index.php?title=25_October_2010&action=edit 25] [http://2010.igem.org/wiki/index.php?title=26_October_2010&action=edit 26] [http://2010.igem.org/wiki/index.php?title=27_October_2010&action=edit 27] [http://2010.igem.org/wiki/index.php?title=28_October_2010&action=edit 28] [http://2010.igem.org/wiki/index.php?title=29_October_2010&action=edit 29] [http://2010.igem.org/wiki/index.php?title=30_October_2010&action=edit 30] [http://2010.igem.org/wiki/index.php?title=31_October_2010&action=edit 31]
== Electrophoretic run (Practice)==

Time: From 9:00 to 18:00
[Member] Iwaki, Fukuyama, Takeuchi, Usui, Adachi
[Details of Experiment]
(1)We made 1% Agarose Gel by using Agarose 0.2g and TEA 20μL.
(2)We mixed DNA 5μL, Marker 1μL and Loading Buffer 1μL.
We applied each 5μL of them to gel.We applied Marker 5μL to the far left lane.
(3) 100V, 30min electrophoresed
(4)Gel soaked in EtBr for 20min.
(5) Band Shooting.




Retrieved from "http://2010.igem.org/11_August_2010"